DETEKSI SIMULTAN CMV DAN CHIVMV PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI DENGAN DUPLEX RT-PCR
| Main Authors: | Chandra, I Gede Agus Adi; Program Magister Bioteknologi Pertanian, Program Pascasarjana Universitas Udayana, Nyana, I Dewa Nyoman; Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Udayana, Wirya, I G N Alit Susanta; Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Udayana, Suastika, Gede; Laboratorium Virologi Tumbuhan, Institut Pertanian Bogor |
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| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of Agricultural Science and Biotechnology
, 2016
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| Subjects: | |
| Online Access: |
http://ojs.unud.ac.id/index.php/JASB/article/view/21680 |
Daftar Isi:
- Detect the presence virus causes mosaic symptoms by CMV and ChiVMV infecting chili pepper plants at the same time was conducted. There needs a study that aims to detect the associations of both viruses causes mosaic symptoms by single or double infection and simplify the detection process which is necessary for a long and expensive process. The used method is a duplex RT-PCR in hopes of simultaneously detecting two viruses causes mosaic disease. Based on the results of the study showed that the duplex RT-PCR method can detect double infection of CMV and ChiVMV on chili plants and this method more effective to detect two viruses simultaneously in a single PCR reaction.