AKTIVITAS ANTIOKSIDAN TOTAL FLAVONOID DAN FENOL KULIT BATANG GAYAM (Inocarpus fagiferus Fosb)
| Main Authors: | Santi, Sri Rahayu, Sukadana, I Made |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of Chemistry
, 2015
|
| Subjects: | |
| Online Access: |
http://ojs.unud.ac.id/index.php/jchem/article/view/16220 |
Daftar Isi:
- These researchs aim to know of contain total flavonoid, total phenol and antioxidant activity in vitro of gayam stem bark of each extract (Inocarpus fagiferus Fosb). The result of extraction 1 kg stem bark of gayam with 8500 mL ethanol (5 x 1700 mL) to yield 30 g of concentrated extract its brown red. The partition of 15 g concentrated ethanol extract with chloroform, n-buthanol, and water to yields of 1.5g yellowish concentrated chloroform extract, 5.87 g brown red concentrated n-buthanol extract and 5.63 g concentrated water extract respectively. The result of phytochemical test of fourth extract shown that the ethanol, n-buthanol, and water extracts qualitatively contain of flavonoid and phenol compounds, while the chloroform extract only to contain phenol compound with much total flavonoid in ethanol extract 0.14 % and less at n-buthanol extract is 0.09 %. The result of antioxidant activity with DPPH method shown that water and n-buthanol extract of gayam stem bark have value IC50 was 20 ppm smaller than IC50 of vitamin E was 25 ppm its mean the water and n-buthanol extract of gayam stem bark have the highest potent scavenger of free radical. The result of antioxidant activity with lipid peroxide-ammonium thiocyanate method shown the extract n-buthanol extract have the highest potent inhibition of formed lipid peroxide (63.04%), it is higher than antioxidant activity of vitamin E (50.39%).