KAPASITAS ANTIOKSIDAN SENYAWA GOLONGAN TRITERPENOID PADA DAUN PRANAJIWA (Euchresta horsfieldii lesch benn)
Main Authors: | Ayu Intan Sari, Kadek, Gede Gunawan, I Wayan, Dharma Putra, Ketut Gede |
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Format: | Article application/pdf eJournal |
Bahasa: | eng |
Terbitan: |
Journal of Chemistry
, 2015
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Subjects: | |
Online Access: |
http://ojs.unud.ac.id/index.php/jchem/article/view/15250 |
Daftar Isi:
- Isolation, identification, and anti-oxidant assay of triterpenoid compound from Euchresta horsfieldii lesch benn) have been conducted. Maseration of 600 g pranajiwa leaves powder with n-hexane produced 6,1 g n-hexane extract which gave yellow to purple colour change with Lieberman-Burchard reagent. The separation with column chromatography using silica gel 60 obtained 3 fractions (F1, F2, F3) which were then analyzed with triterpenoid test. The positive fraction of triterpenoid (F1) produced 2 g yellow isolate. Infrared spectra indicated that the isolate was a triterpenoid, with characteristic functional groups of OH, –CH, C=O, –C=C, –CH2, –CH3, and C–O alcohol. Ultraviolet-visible spectra showed broad peak at 217 nm. The result of anti-oxidant capacity test of triterpenoid was 94,67%, showing that triterpenoid from pranajiwa leaves can be use as an anti-oxidant.