UJI TOKSISITAS EKSTRAK DAUN WARU (Hibiscus tiliaceus L.) TERHADAP LARVA Artemia salina Leach SERTA IDENTIFIKASI GOLONGAN SENYAWANYA
| Main Authors: | Rustini, Ni Luh, Ariati, Komang, Indah Purna Dewi, A. A., Dira Swantara, I Made |
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| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of Chemistry
, 2015
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| Subjects: | |
| Online Access: |
http://ojs.unud.ac.id/index.php/jchem/article/view/15248 |
Daftar Isi:
- The “Brine Shrimp Lethality Test” (BSLT) is a preliminary test used to monitor the bioactive compounds from natural products as anti-cancer. This research aimed to determine of toxic compounds in the leaf of waru (Hibiscus tiliaceus L.). Maceration of 900 g waru leaf powder with 7000 mL of ethanol (5x24 hours) yielded 53,19 g ethanol extract with toxicity tests to larvae of Artemia salina Leach (LC50) of 79.43. The ethanol extract was partitioned to yield 1,81 g of n-hexane (LC50 = 63,09 ppm), 1,52 g of chloroform (LC50 = 1000 ppm) and 50,08 g of aqueous extract (LC50 = 316 ppm). The most toxic, n-hexane extract, was then separated , purified and identified. n-hexane extract was separated by column chromatography with silica gel 60 as stationary phase and n-hexane-chloroform-ethanol (5:4:1) as mobile phase to yield 7 fractions. Fraction one (F1) with a single stain on thin-layer chromatography was the most toxic (LC50 = 398 ppm). Identification with phytochemical test, UV-Vis spectrophotometer and IR spectrophotometer, showed that the isolate contained sterol compounds.