Identifikasi Senyawa Kimia Minyak Sereh Wangi Klon G3 (Cymbopogon nardus L.) dengan Media Tanam Tanah Gambut dan Potensinya Sebagai Antibakteri Enterococcus faecalis
| Main Authors: | udawaty, wis, Yusro, Fathul, Sisillia, Lolyta |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Tanjungpura
, 2020
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| Online Access: |
http://jurnal.untan.ac.id/index.php/tengkawang/article/view/36835 http://jurnal.untan.ac.id/index.php/tengkawang/article/view/36835/75676585222 |
Daftar Isi:
- Plants as an alternative medicinal becomes an option to cope of the synthetic antibiotic resistance to disease-causing bacteria. Essential oil of citronella (Cymbopogon nardus L.) contains antibacterial compounds that citronellal, geraniol and citronellol obtained through steam distillation and water. E. faecalis is among bacteria develops resistance to antibiotics. This experimental reasearch is aimed to test the antibacterial activity of citronella oil were planted in peat soils from West Kalimantan againts E. faecalis bacteria and to identify chemical compounds of citronella oil quantitatively by Gas Chromatography-Mass Spectometry (GCMS) analysis. Testing of antibacterial activity of citronella oil using disc diffusion method in MHA with concentrations of 5%, 10%, 15% and 20% and be repeated 3 times. The controls used amoxicillin and amikacin (positive control) and DMSO (negative control). The research result obtained was fourth of concentration test has a response barriers against E. faecalis with an average diameter category sequentially 10.667 ± 1.155 mm; 15.333 ± 0.577 mm; 20.667 ± 1.155 mm; 24.667 ± 0.577 mm. GCMS analysis identified that citronella oil has 26 chemical compounds. Three major compounds with the largest percentage areas are geraniol (31.65%), citronellal (19.42%) and citronellol (15.56%). It was concluded that citronella oil contains antibacterial compounds and the most effective against E. faecalis in a concentration of 20%.Keywords: antibacterial, E. faecalis, GC-MS, citronella oil, steam distillation and water