PENURUNAN AKTIVITAS FAGOSITOSIS SEL MAKROFAG MENCIT SETELAH DISTIMULASI MINYAK ATSIRI KENCUR TERHADAP ACTINOBACILLUS ACTINOMYCETEMCOMITANS
| Main Author: | Haniastuti, Tetiana |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran Gigi USU
, 2016
|
| Online Access: |
https://jurnal.usu.ac.id/index.php/dentika/article/view/8798 https://jurnal.usu.ac.id/index.php/dentika/article/view/8798/3789 |
Daftar Isi:
- Macrophage plays an essential role in innate immune system by phagocytosing bacteria. However, the phagocytosis process may activate macrophage to release inflammatory mediators which may cause tissue destructions in strong reaction. Kaempheria galanga L. is one of medical plants which is believed having potency as an immunomodulator agent. The aim of this study was to examine macrophage cells phagocytic activity of Actinobacillus actinomy-cetemcomitans after stimulated with Kaempheria galanga`s essential oil in vitro. Mouse peritoneal macrophages were incubated with 5%, 10%, and 20% of Kaempheria galanga`s essential oil at 37oC in an atmosphere containing 5% CO2. After 15 minutes, A. actinomycetemcomitans was added to the suspension and incubated for 30 minutes. At the end of the incubation period, the cells were assayed for the phagocytic activity. The result of this study showed that Kaempheria galanga`s essential oil significantly reduced the percentage of mouse macrophage cells phagocytosing A. actinomy-cetemcomitans and the number of phagocytosed A. actinomycetemcomitans per 1 macrophage cell in vitro (p<0,05). In conclusion, Kaempheria galanga`s essential oil may reduce mouse macrophage cells phagocytic activity of A. actinomycetemcomitans. Key words: macrophage, phagocytosis, Kaempferia galanga linneus