INCREASING OF GLYCOSAMINOGLYCAN AND HYDROXYPROLINE CONTENT IN RAT CARTILAGE MANDIBLES AFTER INSULIN LIKE GROWTH FACTOR–I INDUCTION
| Main Author: | Wahju Ardani, I Gusti Aju |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran Gigi USU
, 2015
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| Online Access: |
https://jurnal.usu.ac.id/index.php/dentika/article/view/2984 https://jurnal.usu.ac.id/index.php/dentika/article/view/2984/1475 |
Daftar Isi:
- Abstract The cellular effects of Insulin Like Growth Factor-I (IGF-I) are mediated by binding to cell IGF-I receptors, which are found mainly on cells of mesenchymal origin, such of fibroblast, chondrocytes, and osteoblast. The study suggests that IGF-I may regulate chondrocytes proliferation, differentiation and extracellular matrix deposition. The purpose of this study was to quantify a synthesis of hydroxyproline HYP and glycosaminoglycan (GAG) by primary chondrocyte culture with IGF-I at 0, 5, 25, and 50 ng/ml concentration. Chondrocyte proliferation was assessed with collagen synthesis and measured by using hydroxyproline (HYP) assay and glycosaminoglycan GAG synthesis was determined with dimethylmethylene blue dye binding assay for 10 days. Primary chondrocytes was cultured for 10 days with 0, 5, 25 and 25ng/ml IGF-I. The results showed that, IGF-I was a potent in increasing GAG synthesis and also effective in promoting collagen HYP synthesis. At the 50 ng/ml concentration, IGF-I was upregultators of GAG synthesis, producing 6 times more GAG than control. Collagen content was promoted by IGF-I at its lower concentration, with level 3 times higher than control. In conclusion, IGF-I at doses ranging from 5 to 50 ng/ml for ten days has proved the induction GAG and HYP synthesis in 10 days primary chondrocyte culture of secondary cartilage rat condyles. Key words: primary chondrocyte culture, glycosaminoglycan, hydroxyproline Abstrak Efek selular Insulin Like Growth Factor-I (IGF-I) diperantarai melalui ikatan IGF-I reseptor, yang terutama ditemukan pada sel asal yaitu sel mesenkim, seperti fibroblast, kondroblast, dan osteoblast. Penelitian ini menunjukkan IGF-I dapat mengatur proliferasi kondrosit, diferensiasi dan deposit ekstraselular matiks. Tujuan penelitian kuantitatif ini adalah mengukur sintesis kolagen dengan kultur primer kondrosit dan IGF-I pada konsentrasi 0, 5, 25, and 50 ng/ml. Proliferasi kondrosit dihitung dengan sintesis kolagen dan diukur menggunakan sintesis hydroxyproline HYP assay and glycosaminoglycan GAG, yang ditentukan dengan dimethylmethylene blue dye binding assay pada hari ke-10. Kultur primer kondrosit dikultur selama 10 hari dengan IGF-I pada konsentrasi 0, 5, 25, and 50 ng/ml. Hasil penelitian menunjukkan bahwa IGF-I potensial dalam meningkatkan sintesis GAG dan sintesis kolagen HYP. Pada konsentrasi 50 ng/ml, IGF-I meningkatkan sintesis GAG, produksi GAG enam kali lebih tinggi dari kontrol. Jumlah kolagen dipromosikan melalui IGF-I pada konsentrasi yang rendah, dengan tingkat tiga kali lebih tinggi dari kontrol. Sebagai kesimpulan, IG-I dengan dosis dari 5 sampai 50 ng/ml selama 10 hari terbukti dapat menginduksi sintesis GAG dan HYP dalam 10 hari kultur primer kondrosit dari kartilago sekunder kondili mandibula tikus. Kata kunci: kultur kondrosit primer, glikosaminoglikan, hidroksiprolin