Pengaruh Pemberian Ekstrak Purwoceng (Pimpinella alpina Molk) terhadap Peningkatan Indikator Vitalitas Pria Studi Eksperimental pada Tikus Jantan Sprague Dawley
| Main Author: | Nasihun, Taufiqurrachman; Bagian Biokimia/Andrologi Fakultas Kedokteran Universitas Islam Sultan Agung Semarang. |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran, Universitas Islam Sultan Agung (UNISSULA)
, 2012
|
| Subjects: | |
| Online Access: |
http://sainsmedika.fkunissula.ac.id/index.php/sainsmedika/article/view/43 http://sainsmedika.fkunissula.ac.id/index.php/sainsmedika/article/view/43/28 |
Daftar Isi:
- Background. Pimpinella alpina Molk (PAM) or ?purwoceng? has been traditionally used as an alternativetreatment to enhance male vitality. Unfortunatelly, the effect of PAM to enhance vitallity which is indicated byincreasing in consentration of Te, LH, and FSH has not been fully understood. This study was conducted toelucidate whether treatment of PAM crude extract will be able to enhance vitality which is indicated withincreasing in level of testosteron, LH, and FSH.Design and Method: In the Post Test Control Group Design, 30 male of three month-old Sprague Dawley ratsrandomly assigned into three groups, i.e Group A as a control, Group B, and group C was treated orally withaquadest 2 ml, 1 ml (25 mg), and 2 ml (50 mg) of PAM crude extract respectively for seven days. tetestosteron,LH, and FSH level were assessed by RIA and IRMA respectively. The different level of testosteron, LH, and FSHamong the three groups was analyzed by ANOVA, followed by Post Hoct Tukey HSD.Result: The result showed significantly higher serum testosterone and LH level (p=0.049 and p=0.853respectively) in 50 mg treated rats when compared with control while no significant difference was seen in theserum levels of these two markers between 25 mg treated rats and 50 mg treated rats. Statistical comparisonrevealed no significant difference in FSH level either in the two treated groups as compared to control or 25mgtreated group to 25 mg treated group.Conclusion: Treatment of extract PAM 50 mg could enhance vitality which is indicated with increasing intestosterone and LH level, but had no effect to FSH level, (Sains Medika, 1 (1) : 53-62).Pendahuluan: Pimpinella alpina Molk (PAM) atau purwoceng telah digunakan sebagai obat alternatif secaraturun temurun untuk meningkatkan vitalitas pria. Namun, pengaruh ekstrak PAM terhadap peningkatan vitalitasdengan indikator peningkatan kadar Te, LH, dan FSH masih belum jelas. Penelitian ini bertujuan untukmengetahui apakah pemberian ekstrak PAM mampu meningkatkan vitalitas dengan indikator peningkatankadar testosteron, LH, dan FSH.Metode Peneletian: Post Test Control Group Design dengan sampel 30 tikus jantan Sprague Dawley umur 3bulan dibagi menjadi 3 group secara random masing-masing terdiri dari 10 ekor tikus. Group A, sebagai kontroldiberi aquadest 2 ml, Group B dan Group C masing-masing diberi 1 ml ekstrak PAM (25 mg) dan 2 ml ekstrakPAM (50 mg). Setelah satu minggu aklimatisasi tikus diberi perlakuan selama 7 hari berturut-turut denganacufirm (blunt type needle). Te kemudian diperiksa dengan metode Radio Immuno Assay (RIA), sehingga Teyang diukur adalah Te total (TTe), sedangkan LH dan FSH diperiksa dengan metode Immuno Radiometric Assay(IRMA).Kadar testosteron, LH, dan FSH, masing-masing diuji dengan metode RIA dan IRMA. Perbedaan kadartestosteron, LH, dan FSH di antara group dianalisis dengan ANOVA dan dilanjutkan dengan uji Post Hoct TukeyHSD.Hasil Penelitian: Hasil penelitian menunjukkan bahwa group C mempunyai rerata kadar testosteron lebihtinggi bermakna dibanding group A, namun tidak bermakna dibanding group B. Rerata kadar testosterongroup B lebih tinggi dibanding group A namun tidak bermakna. Rerata kadar LH group C lebih tinggi bermakna dibanding group A, namun tidak bermakna dibanding group B. Rerata kadar LH group B lebih tinggi dibandinggroup A namun tidak bermakna. Rerata kadar FSH group C lebih tinggi dibanding group A dan group B namuntidak bermakna, demikian pula dengan group B dibanding group A.Kesimpulan: Pemberian ekstrak PAM 50 mg mampu meningkatkan vitalitas dengan indikator peningkatankadar Testosteron dan LH, namun tidak meningkatkan kadar FSH, (Sains Medika, 1 (1) : 53-62).