Sensitivitas dan Spesifisitas Pemeriksaan Imunokromatografi Tuberkulosis Dibandingkan dengan Kultur Lowenstein-Jensen
| Main Authors: | Jannah, Della, Rahmawati, Indah, Rujito, Latip |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran, Universitas Islam Sultan Agung (UNISSULA)
, 2012
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| Subjects: | |
| Online Access: |
http://sainsmedika.fkunissula.ac.id/index.php/sainsmedika/article/view/26 http://sainsmedika.fkunissula.ac.id/index.php/sainsmedika/article/view/26/14 |
Daftar Isi:
- Background: Tuberculosis remains major cause of morbidity and mortality in the world. Approximately one third of the world?s population is infected with Mycobacterium tuberculosis, and it is estimated that 8 million new cases occur each year. It is difficult to diagnose TB in early phase because the symptoms are not specific. The gold standard for TB diagnosis is the culture of M. tuberculosis. It can be performed on a variety of specimens. It is much more sensitive than microscopy but requires more qualified personnel and takes a longer time to provide results. One alternative to support tuberculosis diagnosis is immunochromatographic tuberculosis (ICT Tuberculosis) test. The ICT Tuberculosis test is a test based on the detection of immunoglobulin G (IgG) antibodies directed against antigens secreted by Mycobacterium tuberculosis during active infection. Design and Method: This research used diagnostic test with cross sectional approach. Sampling technique used was a consecutive sampling. Sputum and sera from 27 patients suspected of having pulmonary tuberculosis who visit to Klinik Paru RSUD Prof. Dr. Margono Soekarjo Purwokerto were obtained. ICT Tuberculosis test?s results were compared with sputum culture. Result: The sensitivity and specificity of the ICT Tuberculosis test were 55,6% and 100%,? respectively. Conclusion: The analysis results in this research showed that ICT Tuberculosis test could not be used to diagnosis TB. Pendahuluan: Tuberkulosis (TBC) masih menjadi penyebab kesakitan dan kematian di dunia. Sepertiga dari populasi dunia terinfeksi oleh Mycobacterium tuberculosis, dan diperkirakan 8 juta kasus baru terjadi setiap tahun. Diagnosa TBC pada tahap awal sangat sulit dilakukan karena gejala tidak spesifik. Standard emas untuk diagnosa TBC adalah kultur Mycobacterium tuberculosis pada berbagai spesimen karena jauh lebih sensitif dibandingkan mikroskopis, tetapi memerlukan kualifikasi personil dan waktu yang lebih panjang untuk memberikan hasil. Salah satu alternatif untuk mendukung diagnosa TBC adalah Tes Immunochromatographic TBC (Tuberculosis ICT). Tes ICT berdasar pada deteksi immunoglobulin G (IgG) terhadap antigen yang disekresi oleh Mycobacterium tuberculosis aktif selama infeksi. Metode Penelitian: Penelitian ini menggunakan uji diagnostik dengan pendekatan cross sectional. Teknik sampling yang digunakan adalah consecutive sampling. Dahak dan serum dari 27 pasien yang diduga memiliki TBC paru diperoleh dari pasien yang datang ke Klinik Paru RSUD Prof. Dr. Margono Soekarjo Purwokerto. Hasil uji ICT TBC dibandingkan dengan kultur dahak. Hasil Penelitian: Sensitifitas dan spesifisitas dari Tes ICT TBC yang diperoleh adalah 55,6% dan 100%. Kesimpulan: Analisis hasil penelitian ini menunjukkan bahwa Tes ICT TBC tidak dapat digunakan untuk diagnosa TBC. ?r"?C???rif"; mso-fareast-font-family:"Arial Unicode MS";mso-bidi-font-family:Tahoma; mso-ansi-language:EN-GB'>Conclusion: The analysis results in this research showed that ICT Tuberculosis test could not be used to diagnosis TB. ? Key words: culture of M. tuberculosis, diagnostic test, Immunochromatographic tuberculosis