Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D
| Main Authors: | Hudaya, Isna; Department of Midwifery, Medical Faculty, Sultan Agung Islamic University Raya Kaligawe Street KM. 4. Semarang, Central Java Indonesia, Nasihun, Taufiq R; Department of Biomedical Sciences, Medical Faculty, Sultan Agung Islamic University Raya Kaligawe Street KM. 4. Semarang, Central Java Indonesia, Sumarawati, Titiek; Department of Biomedical Sciences, Medical Faculty, Sultan Agung Islamic University Raya Kaligawe Street KM. 4. Semarang, Central Java Indonesia |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran, Universitas Islam Sultan Agung (UNISSULA)
, 2016
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| Subjects: | |
| Online Access: |
http://sainsmedika.fkunissula.ac.id/index.php/sainsmedika/article/view/192 http://sainsmedika.fkunissula.ac.id/index.php/sainsmedika/article/view/192/159 |
Daftar Isi:
- Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE).Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 μg/mL; 500 μg/mL; 250 μg/mL; 125 μg/mL; 62.5 μg/mL; 31.25 μg/mL; 15.62 μg/mL; 7.81 μg/mL; 3.90 μg/mL; 1.95 μg/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis.Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 μg/ml and 13.71 μg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (α = 0.05), meaning that there was significant relationship.Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.