RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS
| Main Authors: | Wasito, R., Wuryastuti, Hastari |
|---|---|
| Format: | Article info eJournal |
| Terbitan: |
Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI
, 2015
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| Subjects: | |
| Online Access: |
https://journal.ugm.ac.id/jsv/article/view/8625 |
Daftar Isi:
- Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopathology diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to localize rabies virus antigen in formalin- fixed tissues. The results of the present study also demonstrated that a procedures using formalin-fixed parafin-embeded tissue and stained immunopathologicalH by the ABC method was an excellent method for both preservation of morfological details of ceils/tissues and demonstration of rabies virus antigen.