A Study of Macroscopic and Microscopic Lesions of Chicken Embryos Infected by Virulent Newcastle Disease Virus Field Isolates
| Main Authors: | Putra, Hamdu Hamjaya, Wibowo, Haryadi, Untari, Tri, Kurniasih, Kurniasih |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI
, 2012
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| Online Access: |
https://journal.ugm.ac.id/jsv/article/view/2468 https://journal.ugm.ac.id/jsv/article/view/2468/2210 |
Daftar Isi:
- Newcastle disease (ND) is caused by Avian paramyxovirus, family Paramyxoviridae, one of the major diseases in chickens. This research was aimed to find lesions in chicken's embryo organs macroscopically andmicroscopically, infected by pathogenic ND virus. Embryonic chicken eggs (ECE) were inoculated by the ND Salatiga virus and ND La Sota virus as a control avirulent virus. Aquabidestilata used as a negative control. ECEwich showed the death of the embryos, stored in the refrigerator. The allantois fluid was collected, for further examination of viral growth. Chicken embryos that died then observed macroscopically. The organs of chicken embryos were made into histopathologic preparations stained with Hematoxylin and Eosin (H&E) for microscopic analysis. The identification of ND virus growth on isolates was done by haemagglutination andhaemagglutination inhibition test using an anti-ND serum. The chicken embryos that were infected by the ND Salatiga virus died approximately 26 hours post-inoculation. Macroscopic lesions were visible as haemorrhagein the skin. Microscopic lesions indicated the congestion and haemorrhage in lungs, inflammation and congestion in the skin, congestion in intestines, liver, kidneys and heart. There was also mild congestion on theskin in chicken embryos infected by ND La Sota virus. The microscopic lesions showed congestion in lungs, liver, kidneys and heart, also the inflammation and congestion on the skin. The macroscopic and microscopic lesions of chicken embryos infected by the ND Salatiga virus were more severe than lesions caused by ND La Sota virus.Key words: Newcastle disease, chicken embryos, macroscopic lesions, microscopic lesions, La Sota
- Newcastle disease (ND) disebabkan oleh Avian paramyxovirus dari keluarga Paramyxoviridae, merupakan salah satu penyakit utama pada ayam. Penelitian ini bertujuan untuk mengetahui lesi pada organembrio ayam secara makroskopis maupun mikroskopis yang diinfeksi oleh virus ND. Telur ayam berembrio (TAB) diinokulasi oleh virus ND Salatiga dan virus ND La Sota. Aquabidestilata digunakan sebagai kontrolnegatif. TAB yang menunjukkan kematian embrio disimpan di refrigerator, kemudian dikoleksi cairan allantoisnya. Embrio ayam yang mati dilakukan pengamatan secara makroskopis. Organ dari embrio ayam dibuat preparat histopatologi dengan pewarnaan Hematoxylin dan Eosin (H&E) untuk pemeriksaan mikroskopis. Identifikasi adanya pertumbuhan virus ND pada isolat dilakukan dengan uji hemaglutinasi dan uji hemaglutinasi inhibisi menggunakan serum anti ND. Embrio ayam yang diinfeksi oleh virus ND Salatiga mengalami kematian kurang lebih 26 jam pasca inokulasi. Lesi makroskopis yang teramati berupa hemoragipada kulit. Lesi mikroskopis menunjukkan adanya kongesti dan hemoragi pada paru-paru, kongesti dan radang pada kulit, serta kongesti pada usus, hati, ginjal, dan jantung. Embrio ayam yang diinfeksi virus ND La Sota secara makroskopis teramati kongesti ringan pada kulit. Lesi mikroskopisnya menunjukkan adanya kongesti pada paru-paru, kongesti dan radang pada kulit, serta kongesti pada hati, ginjal, dan jantung. Lesi makroskopis dan mikroskopis embrio ayam yang diinfeksi virus ND Salatiga lebih parah bila dibandingkan dengan lesi akibat virus ND La Sota.Kata kunci: Newcastle disease, embrio ayam, lesi makroskopis, lesi mikroskopis, La Sota