GHR/HindIII Locus Polymorphisms in Intron-2 GHR Gene of Papua Local Chicken
| Main Authors: | Mu'in, M. A., Murwanto, A. G. |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Bengkulu
, 2021
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| Subjects: | |
| Online Access: |
https://ejournal.unib.ac.id/jspi/article/view/18045 https://ejournal.unib.ac.id/jspi/article/view/18045/9139 |
Daftar Isi:
- This study aimed to detect single nucleotide polymorphisms (SNPs) in intron-2 on growth hormone receptor (GHR) gene in Papua local chickens using the PCR-RFLP method to study its relationship with growth characteristics. Data on the bodyweight of 49 chickens aged 1, 2, 3, and 4 months (22 males, 27 females) and DNA samples were used for this study. The DNA fragment of size 718 bp in intron-2 of the GHR gene from the study chicken was successfully amplified using a pair of specific primers. The PCR-RFLP/HindIII analysis results found this locus's two genotypes (HindIII++ and HindIII--). HindIII+ and HindIII- alleles were 0.02 and 0.98, respectively.