Kajian Gula Reduksi Menjadi Alkohol dari Tandan Kosong Kelapa Sawit Dengan Sakarifikasi dan Fermentasi Serentak (SSF)
| Main Authors: | Rini Hartari, Widia , Maryanti, Maryanti, Undadraja, Bigi |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Politeknik Negeri Lampung
, 2021
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| Online Access: |
https://jurnal.polinela.ac.id/jppt/article/view/1878 https://jurnal.polinela.ac.id/jppt/article/view/1878/1318 |
Daftar Isi:
- Alcohol is closely related to daily life. It is used as a solvent or additional substance in the food, industry, pharmacology, laboratory, and beauty sectors. Empty oil palm bunches waste is the potential to process into alcohol because it contains cellulose that can be transformed into sugar, and the sugar is fermented into alcohol. The objective of this research was to find out the reducing sugar and alcohol produced from empty oil palm bunches. This research was done in two stages. The first stage was the preparation of empty oil palm bunches with basal (NaOH) treatment. The second stage included fermentation with cellulose enzyme (Novozymes Yield CL) for 24 hours and then fermented with Saccharomyces cerevisiae for 72 hours. The substrate concentrations to use were 7.5% and 10% and starter concentrations were 10%, 15%, and 20%. Research data were analyzed by using a completely randomized design (CRD) and then followed with the least significant difference (LSD). The result showed that 7.5% substrate addition produced the highest reducing sugar by 3.014% and then 10% substrate produced 2.686% reducing sugar. The alcohol test result showed that 10% substrate and 20% starter were significantly different by 3.5%, and 7.5% and 10% produced the lowest alcohol by 1%.
- Alkohol sangat erat dalam kehidupan sehari-hari, alkohol digunakan menjadi pelarut atau bahan tambahan dalam bidang makanan, industri, farmasi, laboratorium, dan kecantikan. Limbah Tandan Kosong Kelapa Sawit berpotensi diolah menjadi alkohol, karena mengandung selulosa yang diubah menjadi gula, gula itu yang difermentasi menjadi alkohol. Tujuan dari penelitian ini adalah mengetahui gula reduksi dan alkohol yang dihasilkan dari Tandan Kosong Kelapa Sawit. Penelitian dilakukan melalui 2 tahap, yaitu tahap pertama adalah persiapan sampel TKKS yang sudah di pretreatment basa (NaOH). Tahap kedua pelaksanaan penelitian meliputi fermentasi dengan enzim cellulase (Novozymes Yield CL) selama 24 jam, kemudian fermentasi dengan Saccharomyces cerevisiae Selama 72 jam. Konsentrasi substrat yang digunakan 7,5% dan 10%, konsentrasi starter yang digunakan 10%, 15%, dan 20%. Data hasil penelitian di analisis dengan Rancangan Acak Kelompok Lengkap (RAKL) dengan uji lanjut Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa gula reduksi paling tinggi di penambahan substrat 7,5% yaitu 3,014% dan substrat 10% menghasilkan gula 2,686%. Hasil uji alkohol didapatkan bahwa substrat 10% dan starter 20% berbeda nyata yaitu 3,5%, sedangkan substrat 7,5% dan starter 10% menghasilkan alkohol terkecil 1%. Kata kunci : Akohol, Gula Reduksi, Tandan Kosong Kelapa Sawit.