EFEK SITOTOKSIK EKSTRAK ETANOLIK DAUN DEWANDARU (Eugenia uniflora L.) TERHADAP SEL KANKER PAYUDARA T47D DAN MCF-7 Cytotoxic activity of Dewandaru (Eugenia uniflora L.) ethanolic extracts on T47D and MCF-7 cell lines
| Main Authors: | Supriyati, Nita, Mujahid, Rohmat |
|---|---|
| Format: | Article eJournal |
| Bahasa: | eng |
| Terbitan: |
Badan Penelitian dan Pengembangan Kesehatan
, 2018
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| Subjects: | |
| Online Access: |
http://ejournal.litbang.depkes.go.id/index.php/toi/article/view/7867 |
Daftar Isi:
- ABSTRACTDewandaru (Eugenia uniflora L.) has been reported having several activities such as antioxidant, antimutagenic,antitumor and inhibit DNA polymerase on EVB (Eipstein-Bar Virus). This research aimed to know the cytotoxiceffect of dewandaru leaves extract on breast cancer cell line, T47D and MCF-7. The 70% ethanolic extract ofDewandaru was tested to the breast cancer cell line of T47D and MCF-7 by MTT procedure on the concentrationsare as follows: 31,25; 62,5; 125; 250; 500 μg/ml. The chemical content of the extract was determined byphytochemical screening method. The result showed that cytotoxic activities (IC50 value) of dewandaru extracton breast cancer cell line of T47D was 117μg/mL while on MCF-7 was 155 μg/ml. The phytochemical screeningshowed that dewandaru extract contain chemical compound such as alkaloida, saponin, flavonoide, tanin andpolyfenol. ABSTRAKTanaman dewandaru (Eugenia uniflora L.) telah dilaporkan memiliki aktivitas antioksidan, antimutagenik,antitumor dan mampu menghambat DNA polymerase pada EBV(Eipstein-Bar Virus). Penelitian ini bertujuanuntuk mengetahui efek sitotoksik daun dewandaru (Eugenia uniflora L.) terhadap sel kanker payudara T47Ddan MCF-7. Serbuk daun dewandaru dimaserasi dengan penyari etanol 70%. Ekstrak kemudian diujikanterhadap dua macam sel kanker mengunakan metode MTT dengan konsentrasi 31,25;62,5 ;125 ;250 ;500μg/ml. Untuk mengetahui kandungan golongan kimia, dilakukan skrining fitokimia terhadap ekstrak daundewandaru. Dari uji sitotoksik ekstrak daun dewandaru terhadap sel kanker payudara T47D diperoleh nilaiIC50 sebesar 117 μg/ml sedangkan terhadap sel kanker payuadara MCF-7 diperoleh nilai IC50 sebesar 155 μg/ml. Dari hasil skrining fitokimia diketahui bahwa ekstrak daun dewandaru mengandung alkaloid, saponin,flavonoid, tanin dan polifenol.Kata kunci: Eugenia uniflora L., kanker payudara, T47D dan MCF-7.