Correlation between Cytotoxic Activity on MCF-7 Cell an d Total Steroid Level on Trigonella foenum-graecum L. Korelasi aktivitas sitotoksik pada sel MCF-7 dan kadar steroid total Trigonella foenum-graecum L.
| Main Authors: | Agustini, Kurnia, Sumaryono, Wahono, Fitria, Azri |
|---|---|
| Format: | Article eJournal |
| Bahasa: | eng |
| Terbitan: |
Badan Penelitian dan Pengembangan Kesehatan
, 2011
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| Subjects: | |
| Online Access: |
http://ejournal.litbang.depkes.go.id/index.php/toi/article/view/6520 |
Daftar Isi:
- AbstractTrigonella foenum-graecum (TFG) is one of medicinal plants containing some steroidal sapogenins such asdiosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also alkaloid trigonellin, which has many activityas antidiabetic, estrogenic and also as anti cancer. The aim of this research is to investigate the correlationof its cytotoxic activity on breast cancer cell line, MCF-7, with its total steroid level. Samples was prepared byfractioned the methanolic TFG with ethylacetate, n-hexane and n-buthanol. Each fraction then was separatedby chromatographic coloumn. Cytotoxic activity was investigated using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium) assay on MCF-7 cell, while total steroid level was done using spectrophotometric methods.Results showed that there was no correlation between cytotoxic activity and total steroid level. Thus we canconclude that the cytotoxic effect from TFG is not caused by its steroid compounds.AbstrakTrigonella foenum-graecum L. (TFG) adalah salah satu tumbuhan obat yang mengandung beberapa steroid sapogenin seperti diosgenin, yamogenin, gitogenin, tigogenin, dan trigoneosid, serta alkaloid trigonellin. TFG memiliki aktivitas antidiabetik, estrogenik, dan antikanker. Penelitian ini bertujuan untuk menguji korelasi aktivitassitotoksik TGF pada sel kanker payudara MCF-7 serta kadar steroid total. Ekstrak metanol TGF difraksinasi dengan etil asetat, n-heksan, dan n-butanol. Tiap fraksi dipisahkan dengan kromatografi kolom. Aktivitas sitotoksik pada sel MCF-7 diuji dengan uji MTT [3-(4,5-dimetiltiazol-2-il)2,5-difeniltetrazolium]. Kadar steroid total ditetapkan dengan metode spektrofotometri. Hasil penelitian menunjukkan tidak terdapat korelasi antara aktivitas sitotoksik dengan kadar steroid total. Sehingga dapat disimpulkan bahwa efek sitotoksik TFG bukan merupakan kontribusi dari komponen steroid.