Pengukuran Konsentrasi dan Kemurnian DNA Genom Nyamuk Anopheles barbirostris
Main Authors: | Mustafa, Hasrida; Balai Litbang P2B2 Donggala, Badan Litbang Kesehatan, Kementerian Kesehatan RI, Jl. Masitudju No.58 Labuan Panimba, Labuan, Donggala, Sulawesi Tengah, Indonesia, Rachmawati, Indra; Badan Pengkajian dan Penerapan Teknologi (BPPT), Jalan MH.Thamrin 8, Jakarta 10340, Udin, Yusran; Balai Litbang P2B2 Donggala, Badan Litbang Kesehatan, Kementerian Kesehatan RI, Jl. Masitudju No.58 Labuan Panimba, Labuan, Donggala, Sulawesi Tengah, Indonesia |
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Format: | Article application/pdf eJournal |
Bahasa: | eng |
Terbitan: |
Balai Penelitian dan Pengembangan Pengendalian Penyakit Bersumber Binatang Donggala
, 2017
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Subjects: | |
Online Access: |
http://ejournal.litbang.depkes.go.id/index.php/vektorp/article/view/6251 |
Daftar Isi:
- The purity and concentration of DNA information from some sample are important to measure the degree of contamination. Anopheles barbirostris mosquitoes samples from Kalukutinggu Village was extracted to obtain the genome DNA using PureLink Genomic DNA mini Kits. The purity and concentration of DNA Anopheles barbirostris was measured by using nano spectrophotometer. The results showed that DNA concentration was 17,33 – 34,79 ug/ml, and their purity were 1,90 average. The purity and concentration of the DNA from the samples showed were not contaminated and eligible for the next step, that is DNA amplification