Analisis In Silico Senyawa Aktif Sprirulina platensis sebagai Inhibitor Tirosinase: Fatty Acid Profiles of Tuna Loin (Thunnus albacares) Sprayed by Filtered Smoke during Frozen Storage
| Main Authors: | Pannindriya, Prayoga , Safithri, Mega, Tarman, Kustiariyah |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
, 2021
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jphpi/article/view/33122 http://journal.ipb.ac.id/index.php/jphpi/article/view/33122/21527 |
Daftar Isi:
- Hiperpigmentasi merupakan kondisi ketika melanin diproduksi secara berlebih sehingga menimbulkan bercak-bercak pada kulit. Kondisi ini selain menyebabkan masalah estetika juga meningkatkan produksi reactive oxygen species pada kulit. Salah satu cara menangani hiperpigmentasi adalah melalui inhibisi enzim kunci sintesis melanin yaitu tirosinase. Spirulina platensis adalah mikroalga yang telah banyak digunakan sebagai suplemen maupun obat karena kandungan nutrisinya yang tinggi. Ekstrak S. platensis telah terbukti secara in vitro memiliki kemampuan menginhibisi enzim tirosinase. Penelitian ini bertujuan untuk mencari senyawa aktif pada S. platensis yang berpotensi sebagai inhibitor enzim tirosinase. Senyawa-senyawa aktif yang terdapat pada S. platensis ditelusuri melalui studi pustaka kemudian diseleksi berdasarkan afinitas, toksisitas, dan aturan Lipinski. Analisis penambatan molekuler dilakukan dengan senyawa yang terpilih terhadap enzim tirosinase dengan asam kojat sebagai ligan kontrol. Hasil penambatan molekuler menunjukkan bahwa kaempferol paling berpotensi sebagai inhibitor tirosinase karena memiliki ΔG paling negatif sehingga memiliki afinitas terbesar terhadap enzim tirosinase.
- Hyperpigmentation is a condition when melanin is overproduced, causing spots on the skin. This condition apart from causing aesthetic problems, can also increase the production of reactive oxygen species at the skin. One way to deal with hyperpigmentation is through inhibiting the key enzyme for melanin synthesis which is tyrosinase. Spirulina platensis is a microalgae that has been widely used as a supplement and medicine because of its high nutritional content. S. platensis extract has been proven in vitro to have the ability inhibit ing the tyrosinase enzyme. This study aimed to find active compounds in S. platensis that have the potential as tyrosinase enzyme inhibitors. The active compounds found in S. platensis were explored through literature studies and then selected based on affinity, toxicity, and Lipinski's Rule. Molecular docking analysis was carried out with selected compounds against the tyrosinase enzyme with kojic acid as the control ligand. The results of molecular docking showed that kaempferol has the best potential as tyrosinase inhibitor since t has the most negative ΔG thus has the best affinity with the tyrosinase enzyme.