Uji diagnostik rapid test antigen helicobacter pylori dalam feses dengan metode imunokromatografi terhadap urea breath test pada pasien dispepsia = Diagnostic test of helicobacter pylori stool antigen rapid test using immunochromatography against urea breath test in dyspeptic patients
| Main Authors: | Chyntia Octaviani, author, Add author: July Kumalawati, supervisor, Add author: Murdani Abdullah, supervisor, Add author: Suzanna Immanuel, examiner, Add author: Ina S. Timan, examiner, Add author: Ninik Sukartini, examiner |
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| Format: | Bachelors |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20482967 |
Daftar Isi:
- <b>ABSTRAK</b><br> <p style="text-align: justify;"><strong>Latar Belakang: </strong>Infeksi <em>Helicobacter pylori</em> merupakan infeksi kronis bakterial yang berhubungan dengan penyakit gastroduodenal. Berdasarkan konsensus Bangkok, pemeriksaan diagnostik infeksi <em>H.pylori</em> hendaknya dilakukan pada semua pasien dispepsia kronis. <em>Urea breath test </em>(UBT) merupakan pemeriksaan referens non-invasif dengan biaya cukup mahal. <em>Rapid test</em> antigen feses merupakan pemeriksaan yang praktis dengan biaya lebih terjangkau. Penelitian ini bertujuan mengevaluasi peran diagnostik <em>rapid test</em> antigen <em>H.pylori</em> dalam feses terhadap UBT pada pasien dispepsia.</p><p style="text-align: justify;"><strong>Metode: </strong>Penelitian ini merupakan uji potong lintang terhadap pasien dispepsia di RSUPN Cipto Mangunkusumo selama bulan Agustus-Oktober 2018. Sebanyak 70 subjek diambil secara <em>consecutive sampling</em> dan dilakukan pemeriksaan <em>rapid test SD Bioline H.pylori Ag<sup>®</sup></em> dan Urea [<sup>13</sup>C] Breath Test Kit-Heliforce<sup>®</sup>.</p><p style="text-align: justify;"><strong>Hasil: </strong>Rerata usia subjek penelitian adalah 46,2 ± 14,23 tahun (18-70 tahun) dan terdapat 17,14% subjek positif terinfeksi <em>H.pylori</em> berdasarkan hasil UBT. Sensitivitas, spesifisitas, nilai prediksi positif, dan nilai prediksi negatif <em>rapid test</em> secara berurutan adalah 41,67%, 100%, 100%, dan 89,23%.</p><p style="text-align: justify;"><strong>Simpulan: </strong><em>R</em><em>apid test</em> antigen <em>H.pylori</em> dalam feses memiliki sensitvitas yang kurang baik tetapi memiliki spesifisitas, NPP, dan NPN yang cukup baik; praktis digunakan; dan harganya jauh lebih terjangkau sehingga masih dapat dipertimbangkan untuk digunakan pada daerah dengan keterbatasan ekonomi dan fasilitas.</p><p style="text-align: justify;"><strong>Kata Kunci: </strong>Gastritis; <em>lateral flow immunoassay</em>; <sup>13</sup>C-UBT</p><hr /><p><strong> <hr> <b>ABSTRACT</b><br> Background: </strong><em>Helicobacter pylori</em> infection is a chronic bacterial infection associated with gastroduodenal diseases. Based on the Bangkok consensus, a diagnostic test of <em>H.pylori</em> infection should be carried out in all patients with chronic dyspepsia. Urea breath test (UBT) is a non-invasive reference test with a fairly expensive cost. Stool antigen rapid test is a practical test with a more affordable cost. We aimed to evaluate the diagnostic role of the <em>H.pylori</em> stool antigen rapid test against UBT in dyspeptic patients.</p><p><strong>Methods: </strong>This was a cross-sectional study of dyspeptic patients at RSUPN Cipto Mangunkusumo during August-October 2018. A total of 70 subjects were taken by consecutive sampling method and tested with <em>rapid test SD Bioline H.pylori Ag<sup>®</sup></em> and Urea [<sup>13</sup>C] Breath Test Kit-Heliforce<sup>®</sup>.</p><p><strong>Results: </strong>The mean age of the subjects was 46.2 ± 14.23 years (18-70 years) and there were 17.14% subjects positively infected with <em>H.pylori</em> based on UBT results. Sensitivity, specificity, positive predictive value, and negative predictive value of the rapid test were 41.67%, 100%, 100%, and 89.23% respectively.</p><p><strong>Conclusion: </strong><em>Helicobacter pylori</em> stool antigen rapid test had poor sensitivity but had a good specificity, PPV, and NPV; practical use; and more affordable price so that it could still be considered to be used in areas with economic and facilities limitations.</p><p><strong>Keywords: </strong>Gastritis; lateral flow immunoassay; <sup>13</sup>C-UBT</p>