Respons biologis fibroblas dan osteoblas yang diinduksi dengan cdt like protein aggregatibacter actinomycetemcomitans hasil isolasi dari periodontitis kronis dan periodontitis agresif = Biological responses of fibroblast and osteoblast which were induced by cdt like protein aggregatibacter actinomycetemcomitans isolated from chronic periodontitis and aggressive periodontitis
| Main Author: | Gultom, Ferry Pergamus, author |
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| Format: | Doctoral Bachelors |
| Terbitan: |
, 2013
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| Subjects: | |
| Online Access: |
http://lib.ui.ac.id/file?file=digital/20350876-D1398-Ferry Pergamus Gultom.pdf |
Daftar Isi:
- [<b>ABSTRAK</b><br> Latar belakang : Penyakit periodontal baik periodontitis kronis maupun periodontitis agresif adalah manifestasi klinis dari interaksi agen, yaitu bakteri seperti A.actinomycetemcomitans ( Aa ) dan produknya antara lain Cytolethal Distending Toxin ( CDT ) dengan sel inang di jaringan periodontal seperti sel fibroblas dan osteoblas. Interaksi tersebut dan dampaknya dalam patogenesis penyakit periodontal belum sepenuhnya dipahami. Tujuan penelitian : Untuk mengungkapkan peran CDT-like protein Aa yang diisolasi dari periodontitis kronis dan agresif dalam patogenesis penyakit periodontal, dengan cara memaparkan lisat bakteri yang mengandung CDT-like protein dengan fibroblas dan osteoblas. Dampak dari interaksi tersebut dievaluasi berdasarkan analisis viabilitas dan ekspresi mRNA IL ? 1, IL ? 6, TNF ? α dan RANKL dengan menggunakan metode MTT assay dan Real Time PCR. Metoda : Digunakan tiga macam konsentrasi CDT-like protein dari lisat bakteri Aa hasil isolasi dari periodontitis kronis dan agresif yaitu 2 g / l, 10 g / l dan 20 g / l untuk uji viabilitas fibroblas dan osteoblas hasil kultur. Untuk uji ekspresi mRNA, digunakan konsentrasi 2 g / l CDT-like protein dari lisat bakteri yang sama dan diaplikasikan ke sel fibroblas dan osteoblas Hasil : Terdapat kecenderungan berbeda pada viabilitas dan ekspresi sitokin antara sel fibroblas dan osteoblas setelah diberi pemaparan dengan CDT-like protein Aa, baik yang diisolasi dari periodontitis kronis maupun dari peridontitits agresif.Kesimpulan : CDT-like protein yang diproduksi oleh dua isolat Aa yang berbeda yaitu dari periodontitis kronis dan periodontitis agresif mengakibatkan respons biologis yang berbeda terhadap sel fibroblas dan osteoblas. Hasil ini mencerminkan perbedaan mekanisme patogenesis kedua penyakit periodontal tersebut.; <hr> <b>ABSTRACT</b><br> Background: Periodontal diseases both chronic and aggressive periodontitis are the clinical manifestations of the interactions between agents, such as the bacteria A.actinomycetemcomitans (Aa) and the products Cytolethal Distending Toxin ( CDT ) with host cells in periodontal tissues such as fibroblasts and osteoblasts. Interactions and their impact in the pathogenesis of periodontal disease are not fully understood. Objective: To reveal the role of CDT -like protein Aa isolated from chronic and aggressive periodontitis in the pathogenesis of periodontal disease, by exposing bacterial lysate containing CDT-like protein with fibroblasts and osteoblasts. The impact of these interactions evaluated by analysis of viability and mRNA expression of IL - 1, IL - 6, TNF - α and RANKL using the MTT assay method and Real Time PCR. Method: used three different concentrations of CDT-like proteins from Aa bacterial lysates isolated from chronic and aggressive periodontitis is 2  g /  l, 10  g /  l and 20  g /  l to test the viability of cultured fibroblasts and osteoblasts. To test mRNA expression, used a concentration of 2  g /  l CDTlike protein from the same bacterial lysates and applied to the same fibroblasts and osteoblasts Results: There is a distinct trend in the viability and cytokine expression between fibroblasts and osteoblasts cells after exposure to CDT-like like protein Aa, both isolated from chronic periodontitis and from aggresive peridontitis. Results: CDT-like proteins produced by two different isolates Aa i.e. from chronic periodontitis and aggressive periodontitis cause different biological responses to fibroblasts and osteoblasts. These results reflect differences in the mechanism of pathogenesis of both the periodontal diseases., Background: Periodontal diseases both chronic and aggressive periodontitis are the clinical manifestations of the interactions between agents, such as the bacteria A.actinomycetemcomitans (Aa) and the products Cytolethal Distending Toxin ( CDT ) with host cells in periodontal tissues such as fibroblasts and osteoblasts. Interactions and their impact in the pathogenesis of periodontal disease are not fully understood. Objective: To reveal the role of CDT -like protein Aa isolated from chronic and aggressive periodontitis in the pathogenesis of periodontal disease, by exposing bacterial lysate containing CDT-like protein with fibroblasts and osteoblasts. The impact of these interactions evaluated by analysis of viability and mRNA expression of IL - 1, IL - 6, TNF - α and RANKL using the MTT assay method and Real Time PCR. Method: used three different concentrations of CDT-like proteins from Aa bacterial lysates isolated from chronic and aggressive periodontitis is 2  g /  l, 10  g /  l and 20  g /  l to test the viability of cultured fibroblasts and osteoblasts. To test mRNA expression, used a concentration of 2  g /  l CDTlike protein from the same bacterial lysates and applied to the same fibroblasts and osteoblasts Results: There is a distinct trend in the viability and cytokine expression between fibroblasts and osteoblasts cells after exposure to CDT-like like protein Aa, both isolated from chronic periodontitis and from aggresive peridontitis. Results: CDT-like proteins produced by two different isolates Aa i.e. from chronic periodontitis and aggressive periodontitis cause different biological responses to fibroblasts and osteoblasts. These results reflect differences in the mechanism of pathogenesis of both the periodontal diseases.]