Analisis O6-Metilguanin dalam darah pasien kanker yang menerima Siklofosfamid pada regimen terapi secara kromatografi cair kinerja ultra tinggi-tandem spektrometri massa = Analysis of O6-Methylguanine in cancer's patients blood during administration of Cyclophosphamide by ultra high performance liquid chromatography tandem mass spectrometry
| Format: | Bachelors |
|---|---|
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2013
|
| Subjects: | |
| Online Access: |
http://lib.ui.ac.id/file?file=digital/20346330-S47613-Yesa Crystalia.pdf |
| ctrlnum |
20346330 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Analisis O6-Metilguanin dalam darah pasien kanker yang menerima Siklofosfamid pada regimen terapi secara kromatografi cair kinerja ultra tinggi-tandem spektrometri massa = Analysis of O6-Methylguanine in cancer's patients blood during administration of Cyclophosphamide by ultra high performance liquid chromatography tandem mass spectrometry</title><creator/><type>Thesis:Bachelors</type><place/><publisher>Fakultas Farmasi Universitas Indonesia</publisher><date>2013</date><description>[Siklofosfamid merupakan antineoplastik golongan agen pengalkilasi yang banyak
digunakan dalam kemoterapi kanker. Siklofofamid bekerja dengan mengalkilasi
basa DNA pada posisi N7 guanin menghasilkan N7-metilguanin. Siklofosfamid
bersifat tidak selektif dan dapat mengalkilasi gugus nukleofil lain pada DNA yaitu
O6 guanin menghasilkan O6-metilguanin yang berpotensi menyebabkan mutasi
yang dapat memicu kanker sekunder. Pendeteksian O6-metilguanin sebagai salah
satu contoh alkilguanin dapat menjadi salah satu cara monitoring terapi untuk
menghindari risiko kanker sekunder pada pasien kanker yang diberikan
siklofosfamid. Pada penelitian ini dilakukan analisis O6-metilguanin dalam darah
pasien kanker. DNA sampel diisolasi menggunakan QIAgen DNA Mini Kits, lalu
dihidrolisis menggunakan asam format. Hasil hidrolisis diinjeksikan ke KCKUTSM/
SM dengan kolom C18 Acquity UPLC BEH (1,7 &#956;m, 2,1×100 mm)
menggunakan elusi isokratik, fase gerak asam asetat 0,05% dalam aquabidestasetonitril
(95:5), laju alir 0,3 mL/menit, metode ionisasi ESI+, kuantitasi MRM
166,1>149,1 dan 166,1>134,1, volume injeksi 10,0 &#956;L. O6-metilguanin muncul
pada 1,46 menit. Perhitungan menurut kurva kalibrasi memberikan batas deteksi
1,05 ng/mL dan batas kuantitasi 3,50 ng/mL. Dari 72 sampel yang dianalisis, O6-
metilguanin terdeteksi pada 17 sampel, dan dapat dikuantitasi pada 1 sampel
dengan kadar 5,8680 ng/mL., Cyclophosphamide is one of the alkylating agents that widely used in cancer
chemotherapy. It alkylates N7-guanine on DNA as major target, forming N7-
methylguanine. Cyclophosphamide is not selective and can alkylate other
nucleophilic groups such as O6-guanine forming O6-methylguanine which is
potencial to causes mutation leading to secondary cancer in patients who
administered it. O6-methylguanine detection as one of alkylguanine can be one
way to monitor chemotherapy, therefore secondary cancer risk can be avoided.
This research analyzes O6-methylguanine in cancer patients’ blood. DNA
samples were isolated by QIAgen DNA Mini Kits, and hydrolyzed using formic
acid. The hydrolysate was injected to UPLC-MS/MS, column C18 Acquity UPLC
BEH (1.7 &#956;m, 2.1×100 mm), isocratic elution in 3 minutes, mobile phase
consisted of acetic acid 0.05% in aquabidest - acetonitrile (95:5), flow rate 0,3
mL/min, ionization method ESI+, quantification traces 166.1>149.1 and
166.1>134.1, injection volume 10,0 mL. O6-methylguanine’s peak showed in 1,46
min. Extrapolation from calibration curve data gave limit of detection 1.05
ng/mL and limit of quantitation 3,50 ng/mL. Among 72 analyzed samples, O6-
methylguanine was detected on 17 samples, and could be quantified on 1 sample
at concentration 5.8680 ng/mL.]</description><subject>Cyclophosphamide</subject><identifier>20346330</identifier><source>http://lib.ui.ac.id/file?file=digital/20346330-S47613-Yesa Crystalia.pdf</source><recordID>20346330</recordID></dc>
|
| format |
Thesis:Bachelors Thesis |
| title |
Analisis O6-Metilguanin dalam darah pasien kanker yang menerima Siklofosfamid pada regimen terapi secara kromatografi cair kinerja ultra tinggi-tandem spektrometri massa = Analysis of O6-Methylguanine in cancer's patients blood during administration of Cyclophosphamide by ultra high performance liquid chromatography tandem mass spectrometry |
| publisher |
Fakultas Farmasi Universitas Indonesia |
| publishDate |
2013 |
| topic |
Cyclophosphamide |
| url |
http://lib.ui.ac.id/file?file=digital/20346330-S47613-Yesa Crystalia.pdf |
| contents |
[Siklofosfamid merupakan antineoplastik golongan agen pengalkilasi yang banyak
digunakan dalam kemoterapi kanker. Siklofofamid bekerja dengan mengalkilasi
basa DNA pada posisi N7 guanin menghasilkan N7-metilguanin. Siklofosfamid
bersifat tidak selektif dan dapat mengalkilasi gugus nukleofil lain pada DNA yaitu
O6 guanin menghasilkan O6-metilguanin yang berpotensi menyebabkan mutasi
yang dapat memicu kanker sekunder. Pendeteksian O6-metilguanin sebagai salah
satu contoh alkilguanin dapat menjadi salah satu cara monitoring terapi untuk
menghindari risiko kanker sekunder pada pasien kanker yang diberikan
siklofosfamid. Pada penelitian ini dilakukan analisis O6-metilguanin dalam darah
pasien kanker. DNA sampel diisolasi menggunakan QIAgen DNA Mini Kits, lalu
dihidrolisis menggunakan asam format. Hasil hidrolisis diinjeksikan ke KCKUTSM/
SM dengan kolom C18 Acquity UPLC BEH (1,7 μm, 2,1×100 mm)
menggunakan elusi isokratik, fase gerak asam asetat 0,05% dalam aquabidestasetonitril
(95:5), laju alir 0,3 mL/menit, metode ionisasi ESI+, kuantitasi MRM
166,1>149,1 dan 166,1>134,1, volume injeksi 10,0 μL. O6-metilguanin muncul
pada 1,46 menit. Perhitungan menurut kurva kalibrasi memberikan batas deteksi
1,05 ng/mL dan batas kuantitasi 3,50 ng/mL. Dari 72 sampel yang dianalisis, O6-
metilguanin terdeteksi pada 17 sampel, dan dapat dikuantitasi pada 1 sampel
dengan kadar 5,8680 ng/mL., Cyclophosphamide is one of the alkylating agents that widely used in cancer
chemotherapy. It alkylates N7-guanine on DNA as major target, forming N7-
methylguanine. Cyclophosphamide is not selective and can alkylate other
nucleophilic groups such as O6-guanine forming O6-methylguanine which is
potencial to causes mutation leading to secondary cancer in patients who
administered it. O6-methylguanine detection as one of alkylguanine can be one
way to monitor chemotherapy, therefore secondary cancer risk can be avoided.
This research analyzes O6-methylguanine in cancer patients’ blood. DNA
samples were isolated by QIAgen DNA Mini Kits, and hydrolyzed using formic
acid. The hydrolysate was injected to UPLC-MS/MS, column C18 Acquity UPLC
BEH (1.7 μm, 2.1×100 mm), isocratic elution in 3 minutes, mobile phase
consisted of acetic acid 0.05% in aquabidest - acetonitrile (95:5), flow rate 0,3
mL/min, ionization method ESI+, quantification traces 166.1>149.1 and
166.1>134.1, injection volume 10,0 mL. O6-methylguanine’s peak showed in 1,46
min. Extrapolation from calibration curve data gave limit of detection 1.05
ng/mL and limit of quantitation 3,50 ng/mL. Among 72 analyzed samples, O6-
methylguanine was detected on 17 samples, and could be quantified on 1 sample
at concentration 5.8680 ng/mL.] |
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Universitas Indonesia |
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Perpustakaan Universitas Indonesia |
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KOTA DEPOK |
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JAWA BARAT |
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2022-12-13T09:00:14Z |
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