Ekspresi gen Manganese Superoxide Dismurase WnSOD) pada sel Glioma manusia: tinjauan khusus pada hipoksia sel tumor
| Main Authors: | Novi Silvia Hardiany, author, Add author: Septelia Inawati Wanandi, supervisor, Add author: Mohamad Sadikin, supervisor, Add author: Sri Widia Jusman, examiner, Add author: Purnomo Soeharso, examiner, Add author: Ahmad Ruslan Handoto Utomo, examiner |
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| Format: | Masters Bachelors |
| Terbitan: |
, 2008
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| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20341887 |
Daftar Isi:
- [<b>ABSTRAK</b><br> Metode: Ekspresi gen MnSOD dianalisis dengan membandingkan level mRNA dan aktivitas spesiiik enzim MnSOD pada sel glioma dengan sel lekosit (kontrol). Ekspresi gen HIF-lon dianalisis dengan mebandingkan level mRNA HIP-lor pada sel glioma dengan sel lekosit. Ekspresi MnSOD dan HIF-lor. dideteksi pada 20 pasien glioma menggunakan quanrirarive Real Time RT-PCR untuk kadar relatif mRNA MnSOD dan I-HF-la, serta perneriksaan biokimia untuk mengulcur aktivitas enzim MnSOD. Analisis statistik dengan menggunakan SPSS 16.0. Hasil: Ekspresi gen MnSOD baik mRNA maupun aktivitas spesifik enzim MnSOD pada sebagian besar sampel sol glioma manusia diternukan lebih rendah secara signiilkan (p< 0.01) dibandingkan denan sel lekosit. Sedangkan mRNA HIF-lon pada sebagian besar sel glioma manusia ditemukan lebih tinggi secara signifiken (p< 0.05) dibandingkan dengan sel lekosit. Sebanyak 80 % (16 sampel) menunjukkan mRNA I-IIF-lon yang tinggi, yang berarti terdapat hipoksia pada sel glioma. Dari I6 sampel tersebut, 11 sampel menunjukkan ekspresi mRNA MnSOD yang rendah dan 4 sampe] menunjukkan ekspresi mRNA MnSOD yang tinggi. Kesimpulan: Ekspresi gen MnSOD pada sebagian besar sampel ditemukan rendah. Ekspresi HIF-la yang tinggi menunjukkan terdapai hipoksia pada sebagian besar sampel scl glioma. Terdapat perbedaan ekspresi MnSOD pada kondisi hipoksia sel glioma. <hr> <b>ABSTRACT</b><br> Background: Glioma is one of the most frequently found primary brains tumors in Indonesia. Until now, treatment of the glioma is far from successful due to resistancy and recurrance. Therefore, additional therapy is required, such as gene therapy. MnSOD is antioxidant enzymes which is suggested as tumor suppressor, despite its controversies. Solid tumor such as glioma have low oxygen level in the tissue compare to normal tissues. MnSOD as antioxidant enzyme have potential effects on increased ROS (reactive oxygen species) concentration in hypoxia condition. Therefore, further analysis is needed to explain MnSOD gene expression in hypoxia human gliomal cells. Hypoxia in gliornal cells are suggested to influence tumor cells responses toward radiotherapy. Hypoxia slate can be detected using tissues hypoxic marker, hypoxia inducible factor-lot (HIF- lot). Desaign: Cross sectional Aim: To analyzed MnSOD gene expression, hypoxia condition in human glioma cells by analyzing the gene expression of HIP-lot and to analyzed MnSOD gene expression in hypoxia human gliomal cells. Method: MnSOD gene expression was analyzed by comparing MnSOD mRNA level and enzyme specific activity in glioma cells with leucocytes (control). HIF- lot gene expression was analyzed by comparing HIP-lot mRNA level in glioma cells with leucocytes. Twenty glioma patients were included in this study. Quantitative Real Time RT-PCR was used to analyzed MnSOD and HIP-lo. mRNA level. Biochemistry test was used to analyzed MnSOD enzyme spesific activity. Statistical analysis was performed using SPSS 16.0. Results: MnSOD gene expression at mRNA level and enzyme spcsitic activty in most human glioma samples were significantly lower (p< .0l) than leucocytes. While HIF-lo. mRNA level in most human glioma samples were significantly higher (p< .05) than leucocytes. Eighty percents (16) of the samples showed high I-IIF-lot mRNA level, this mean that glioma samples were in hypoxia state. Among the l6 samples, ll samples showed low MnSOD mRNA level and 4 samples showed high mRNA MnSOD level. This mean that there were differences in MnSOD gene expression in hypoxia human glioma cells. Conclusion: MnSOD gene expression in most human glioma samples were low. High HIF-la mRNA level were found in most of glioma samples, meaning that glioma sample were in hypoxia state. There were differences in MnSOD expression in hypoxia human glioma cells., Background: Glioma is one of the most frequently found primary brains tumors in Indonesia. Until now, treatment of the glioma is far from successful due to resistancy and recurrance. Therefore, additional therapy is required, such as gene therapy. MnSOD is antioxidant enzymes which is suggested as tumor suppressor, despite its controversies. Solid tumor such as glioma have low oxygen level in the tissue compare to normal tissues. MnSOD as antioxidant enzyme have potential effects on increased ROS (reactive oxygen species) concentration in hypoxia condition. Therefore, further analysis is needed to explain MnSOD gene expression in hypoxia human gliomal cells. Hypoxia in gliornal cells are suggested to influence tumor cells responses toward radiotherapy. Hypoxia slate can be detected using tissues hypoxic marker, hypoxia inducible factor-lot (HIF- lot). Desaign: Cross sectional Aim: To analyzed MnSOD gene expression, hypoxia condition in human glioma cells by analyzing the gene expression of HIP-lot and to analyzed MnSOD gene expression in hypoxia human gliomal cells. Method: MnSOD gene expression was analyzed by comparing MnSOD mRNA level and enzyme specific activity in glioma cells with leucocytes (control). HIF- lot gene expression was analyzed by comparing HIP-lot mRNA level in glioma cells with leucocytes. Twenty glioma patients were included in this study. Quantitative Real Time RT-PCR was used to analyzed MnSOD and HIP-lo. mRNA level. Biochemistry test was used to analyzed MnSOD enzyme spesific activity. Statistical analysis was performed using SPSS 16.0. Results: MnSOD gene expression at mRNA level and enzyme spcsitic activty in most human glioma samples were significantly lower (p< .0l) than leucocytes. While HIF-lo. mRNA level in most human glioma samples were significantly higher (p< .05) than leucocytes. Eighty percents (16) of the samples showed high I-IIF-lot mRNA level, this mean that glioma samples were in hypoxia state. Among the l6 samples, ll samples showed low MnSOD mRNA level and 4 samples showed high mRNA MnSOD level. This mean that there were differences in MnSOD gene expression in hypoxia human glioma cells. Conclusion: MnSOD gene expression in most human glioma samples were low. High HIF-la mRNA level were found in most of glioma samples, meaning that glioma sample were in hypoxia state. There were differences in MnSOD expression in hypoxia human glioma cells.]