Delesi 30 pb gen laten membran protein-1 (LMP1) virus Epstein-Barr (EBV) pada penderita karsinoma nasofaring (KNF) di Indonesia = The 30-bp Deletion of Epstein-Barr Virus (EBV) latent membrane protein-1 (LMPI) gene in Indonesia Nasophatyngeal Carcinoma (NPC) patient
| Main Authors: | Sri Murni Asih, author, Add author: Dwi Anita Suryandari, supervisor, Add author: Purnomo Soeharso, supervisor, Add author: Yurnadi, examiner, Add author: Budiman Bela, examiner, Add author: Susyana Tamin, examiner |
|---|---|
| Format: | Masters Bachelors |
| Terbitan: |
, 2008
|
| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20341773 |
| ctrlnum |
20341773 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><type>Thesis:Masters</type><title>Delesi 30 pb gen laten membran protein-1 (LMP1) virus Epstein-Barr (EBV) pada penderita karsinoma nasofaring (KNF) di Indonesia = The 30-bp Deletion of Epstein-Barr Virus (EBV) latent membrane protein-1 (LMPI) gene in Indonesia Nasophatyngeal Carcinoma (NPC) patient</title><creator>Sri Murni Asih, author</creator><creator>Add author: Dwi Anita Suryandari, supervisor</creator><creator>Add author: Purnomo Soeharso, supervisor</creator><creator>Add author: Yurnadi, examiner</creator><creator>Add author: Budiman Bela, examiner</creator><creator>Add author: Susyana Tamin, examiner</creator><publisher/><date>2008</date><subject>Nasopharyngeal Neoplasms --diagnosis.</subject><description>[<b>ABSTRAK</b><br>
Latar Belakang: Virus Epstein~Barr (EBV) merupakan virus dsDNA dan
termasnk dalam famili Herpesviridae. Infeksi EBV dapat berasosiasi dengan
beberapa penyakit seperti karsinoma nasofaring (KNF). Pada penderita KNF, gen
EBV yang diekspresikan adalah gen lain, yaitu EBERs, EBNAI, LMP 1, LMPZA,
dan LMPZB. Dari kesemua gen tersebuf., LMPI dianggap yang berperan penting
dalam proses onkogenesis dan transformasi limfosit B oleh EBV. Dan beberapa
Studi epidemiologi, ditemukan adanya Varian khusus pada gen LMP] berupa
deiesi 30 pb pada bagian C-terminal. Di Indonesia, hingga saat ini belum diketahui
apakah ditemukan delesi 30 pb gen LMPI pada penderita KNF dan bila
ditemukan, apakah delesi tersebut berhubungan dengan patogenesis KNF.
Tujnan: Mengetahui apakah ditemukan delesi 30 pb gen LMP] EBV pada
penderita KNF di Indonesia, dan bila ditemukan berapa frekuensi delesi 30 pb gen
LMPI EBV pada penderita KNF di Indonesia, Serta mengetahui hubungan antara
delesi tersebut dengan status patologi KNF.
Mctodc: Identiiiloasi delesi 30 pb gen LMP! Vi1'l.lS Epstein-Barr dilakukan dengan
metode nested PCR dan hasil PCR divisualisasi dengan elektroforesis
menggunakan gel agarose 2%. Hasil amplifikasi bempa pita DNA berukuran 162
pb untuk gen LMPI yang tidak mengalarni delesi 30 pb, sedangkan pita DNA
berukuran 132 pb untuk gen LMP! yang mengalami delesi 30 pb.
Hasil: Dari 100 sampel penderita KNF yang diidentifikasi, 29 sampel mengalami
delesi 30 pb, 71 sampel tidak mengalami delesi 30 pb, dan 21 sampel mengalami
coexislence varian.
Kesimpulan: Di Jakarta, varian EBV berupa delesi 30 pb gen LMPI ditemukan
dalam frekuensi yang rendah (24%; 29/ 121) bila dibandingkan varian yang tidak
mengalami delesi 30 pb (76%; 92/121). Pada penelitian ini juga ditemukan adanya
coexisrence Varian gen LMPL Berdasarkan uji Fisl1er?s Exact, didapat bahwa niiai
p > 0,05, berarti tidak ada hubungan bermakna antara delesi 30 pb gen LMP]
dengan status patologi KINF.
<hr>
<b>ABSTRACT</b><br>
Background: Epstein-Barr vims (EBV) is a dsDNA virus, member of Herpes
(I-lerpesviridae) family. EBV infection may be associated with several diseases,
one of them is nasopharyngeal carcinoma (NPC). NPC patients expressed EBV
latent gene, they are EBERS, EBNA1, LMPI, LMPZA, and LMPZB. LMPI, in
particular play important roles in epithelial oncogenesis and B lymphocyte
transformation. Several epidemiological studies found specific variant of LMP]
gene detectable as 30-bp deletion of C-tenninal region of LMP] gene. There is
not any report of 30-bp LMP] gene on NPC patients so far and it is still unclear
whether the deletion is associated with NPC pathogenesis.
Purpose: (1) To understand the existence of the deletion of 30-bp LMP1 gene in
Indonesia NPC patients. (2) To determine the frequency of 30-bp deletion of
LMP1 gene and its association with pathological status.
Method: Identification of 30-bp deletion in LMPI gene was done by nested PCR
method. The PCR result was investigated by means of electrophoresis in 2%
agarose gel. The results were determined as 162 bp of DNA band of LMP! gene
(without 30-bp deletion) and 132 bp of DNA band of LMP1 gene (with 30-bp
deletion).
Results: Among 100 identified samples, 29 samples found to have 30-bp deletion,
71 samples doesn?t have 30-bp deletion and 21 samples carry coexistence
variants.
Conclusion: In Indonesia, especially in Jakarta, EBV variant of 30-bp deletion of
LMP1 gene was found in low frequency (21-l»%; 29/ 121) in comparison with
variant without deletion (76%; 92/121). There are variant of LMPI gene mixtures
(coexistence with and without deletion). Analysis of data using Fisher°s Exact test
(p>0,05) showed that there is not significant relationship between 30~bp deletion
of LMPI gene and NPC pathological status., Background: Epstein-Barr vims (EBV) is a dsDNA virus, member of Herpes
(I-lerpesviridae) family. EBV infection may be associated with several diseases,
one of them is nasopharyngeal carcinoma (NPC). NPC patients expressed EBV
latent gene, they are EBERS, EBNA1, LMPI, LMPZA, and LMPZB. LMPI, in
particular play important roles in epithelial oncogenesis and B lymphocyte
transformation. Several epidemiological studies found specific variant of LMP]
gene detectable as 30-bp deletion of C-tenninal region of LMP] gene. There is
not any report of 30-bp LMP] gene on NPC patients so far and it is still unclear
whether the deletion is associated with NPC pathogenesis.
Purpose: (1) To understand the existence of the deletion of 30-bp LMP1 gene in
Indonesia NPC patients. (2) To determine the frequency of 30-bp deletion of
LMP1 gene and its association with pathological status.
Method: Identification of 30-bp deletion in LMPI gene was done by nested PCR
method. The PCR result was investigated by means of electrophoresis in 2%
agarose gel. The results were determined as 162 bp of DNA band of LMP! gene
(without 30-bp deletion) and 132 bp of DNA band of LMP1 gene (with 30-bp
deletion).
Results: Among 100 identified samples, 29 samples found to have 30-bp deletion,
71 samples doesn’t have 30-bp deletion and 21 samples carry coexistence
variants.
Conclusion: In Indonesia, especially in Jakarta, EBV variant of 30-bp deletion of
LMP1 gene was found in low frequency (21-l»%; 29/ 121) in comparison with
variant without deletion (76%; 92/121). There are variant of LMPI gene mixtures
(coexistence with and without deletion). Analysis of data using Fisher°s Exact test
(p>0,05) showed that there is not significant relationship between 30~bp deletion
of LMPI gene and NPC pathological status.]</description><identifier>https://lib.ui.ac.id/detail?id=20341773</identifier><recordID>20341773</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Bachelors |
| author |
Sri Murni Asih, author Add author: Dwi Anita Suryandari, supervisor Add author: Purnomo Soeharso, supervisor Add author: Yurnadi, examiner Add author: Budiman Bela, examiner Add author: Susyana Tamin, examiner |
| title |
Delesi 30 pb gen laten membran protein-1 (LMP1) virus Epstein-Barr (EBV) pada penderita karsinoma nasofaring (KNF) di Indonesia = The 30-bp Deletion of Epstein-Barr Virus (EBV) latent membrane protein-1 (LMPI) gene in Indonesia Nasophatyngeal Carcinoma (NPC) patient |
| publishDate |
2008 |
| topic |
Nasopharyngeal Neoplasms --diagnosis |
| url |
https://lib.ui.ac.id/detail?id=20341773 |
| contents |
[<b>ABSTRAK</b><br>
Latar Belakang: Virus Epstein~Barr (EBV) merupakan virus dsDNA dan
termasnk dalam famili Herpesviridae. Infeksi EBV dapat berasosiasi dengan
beberapa penyakit seperti karsinoma nasofaring (KNF). Pada penderita KNF, gen
EBV yang diekspresikan adalah gen lain, yaitu EBERs, EBNAI, LMP 1, LMPZA,
dan LMPZB. Dari kesemua gen tersebuf., LMPI dianggap yang berperan penting
dalam proses onkogenesis dan transformasi limfosit B oleh EBV. Dan beberapa
Studi epidemiologi, ditemukan adanya Varian khusus pada gen LMP] berupa
deiesi 30 pb pada bagian C-terminal. Di Indonesia, hingga saat ini belum diketahui
apakah ditemukan delesi 30 pb gen LMPI pada penderita KNF dan bila
ditemukan, apakah delesi tersebut berhubungan dengan patogenesis KNF.
Tujnan: Mengetahui apakah ditemukan delesi 30 pb gen LMP] EBV pada
penderita KNF di Indonesia, dan bila ditemukan berapa frekuensi delesi 30 pb gen
LMPI EBV pada penderita KNF di Indonesia, Serta mengetahui hubungan antara
delesi tersebut dengan status patologi KNF.
Mctodc: Identiiiloasi delesi 30 pb gen LMP! Vi1'l.lS Epstein-Barr dilakukan dengan
metode nested PCR dan hasil PCR divisualisasi dengan elektroforesis
menggunakan gel agarose 2%. Hasil amplifikasi bempa pita DNA berukuran 162
pb untuk gen LMPI yang tidak mengalarni delesi 30 pb, sedangkan pita DNA
berukuran 132 pb untuk gen LMP! yang mengalami delesi 30 pb.
Hasil: Dari 100 sampel penderita KNF yang diidentifikasi, 29 sampel mengalami
delesi 30 pb, 71 sampel tidak mengalami delesi 30 pb, dan 21 sampel mengalami
coexislence varian.
Kesimpulan: Di Jakarta, varian EBV berupa delesi 30 pb gen LMPI ditemukan
dalam frekuensi yang rendah (24%; 29/ 121) bila dibandingkan varian yang tidak
mengalami delesi 30 pb (76%; 92/121). Pada penelitian ini juga ditemukan adanya
coexisrence Varian gen LMPL Berdasarkan uji Fisl1er?s Exact, didapat bahwa niiai
p > 0,05, berarti tidak ada hubungan bermakna antara delesi 30 pb gen LMP]
dengan status patologi KINF.
<hr>
<b>ABSTRACT</b><br>
Background: Epstein-Barr vims (EBV) is a dsDNA virus, member of Herpes
(I-lerpesviridae) family. EBV infection may be associated with several diseases,
one of them is nasopharyngeal carcinoma (NPC). NPC patients expressed EBV
latent gene, they are EBERS, EBNA1, LMPI, LMPZA, and LMPZB. LMPI, in
particular play important roles in epithelial oncogenesis and B lymphocyte
transformation. Several epidemiological studies found specific variant of LMP]
gene detectable as 30-bp deletion of C-tenninal region of LMP] gene. There is
not any report of 30-bp LMP] gene on NPC patients so far and it is still unclear
whether the deletion is associated with NPC pathogenesis.
Purpose: (1) To understand the existence of the deletion of 30-bp LMP1 gene in
Indonesia NPC patients. (2) To determine the frequency of 30-bp deletion of
LMP1 gene and its association with pathological status.
Method: Identification of 30-bp deletion in LMPI gene was done by nested PCR
method. The PCR result was investigated by means of electrophoresis in 2%
agarose gel. The results were determined as 162 bp of DNA band of LMP! gene
(without 30-bp deletion) and 132 bp of DNA band of LMP1 gene (with 30-bp
deletion).
Results: Among 100 identified samples, 29 samples found to have 30-bp deletion,
71 samples doesn?t have 30-bp deletion and 21 samples carry coexistence
variants.
Conclusion: In Indonesia, especially in Jakarta, EBV variant of 30-bp deletion of
LMP1 gene was found in low frequency (21-l»%; 29/ 121) in comparison with
variant without deletion (76%; 92/121). There are variant of LMPI gene mixtures
(coexistence with and without deletion). Analysis of data using Fisher°s Exact test
(p>0,05) showed that there is not significant relationship between 30~bp deletion
of LMPI gene and NPC pathological status., Background: Epstein-Barr vims (EBV) is a dsDNA virus, member of Herpes
(I-lerpesviridae) family. EBV infection may be associated with several diseases,
one of them is nasopharyngeal carcinoma (NPC). NPC patients expressed EBV
latent gene, they are EBERS, EBNA1, LMPI, LMPZA, and LMPZB. LMPI, in
particular play important roles in epithelial oncogenesis and B lymphocyte
transformation. Several epidemiological studies found specific variant of LMP]
gene detectable as 30-bp deletion of C-tenninal region of LMP] gene. There is
not any report of 30-bp LMP] gene on NPC patients so far and it is still unclear
whether the deletion is associated with NPC pathogenesis.
Purpose: (1) To understand the existence of the deletion of 30-bp LMP1 gene in
Indonesia NPC patients. (2) To determine the frequency of 30-bp deletion of
LMP1 gene and its association with pathological status.
Method: Identification of 30-bp deletion in LMPI gene was done by nested PCR
method. The PCR result was investigated by means of electrophoresis in 2%
agarose gel. The results were determined as 162 bp of DNA band of LMP! gene
(without 30-bp deletion) and 132 bp of DNA band of LMP1 gene (with 30-bp
deletion).
Results: Among 100 identified samples, 29 samples found to have 30-bp deletion,
71 samples doesn’t have 30-bp deletion and 21 samples carry coexistence
variants.
Conclusion: In Indonesia, especially in Jakarta, EBV variant of 30-bp deletion of
LMP1 gene was found in low frequency (21-l»%; 29/ 121) in comparison with
variant without deletion (76%; 92/121). There are variant of LMPI gene mixtures
(coexistence with and without deletion). Analysis of data using Fisher°s Exact test
(p>0,05) showed that there is not significant relationship between 30~bp deletion
of LMPI gene and NPC pathological status.] |
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