Diagnosis malaria pada sampel urin dengan teknik polymerase chain reaction
| Main Authors: | Jonno Berty Bradly Bernadus, author, Add author: Heri Wibowo, supervisor, Add author: Hendri Astuty, supervisor, Add author: Taniawati Supali, examiner, Add author: J. S. B. Tuda, examiner |
|---|---|
| Format: | Masters Bachelors |
| Terbitan: |
, 2009
|
| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20341278 |
| ctrlnum |
20341278 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><type>Thesis:Masters</type><title>Diagnosis malaria pada sampel urin dengan teknik polymerase chain reaction</title><creator>Jonno Berty Bradly Bernadus, author</creator><creator>Add author: Heri Wibowo, supervisor</creator><creator>Add author: Hendri Astuty, supervisor</creator><creator>Add author: Taniawati Supali, examiner</creator><creator>Add author: J. S. B. Tuda, examiner</creator><publisher/><date>2009</date><subject>Malaria --Diagnosis</subject><description><b>ABSTRAK</b><br>
Malaria merupakan penyakit yang masih menimbulkan masalab kesehatan
Masyarakat Indonesia. Prevalensi malaria di beberapa daerah cukup tinggi dan
menjadikan daerah tersebut endemik malaria. Diagnosis malaria ditegakkan
melalui pemariksaan gejala ktJnis dan penemuan parasit pada pemariksaan darah
seoara mikroskopik. Pemariksaan mikroskopik masih merupakan "Gold
Standard", tetapi masih terdapat beberapa kendala dalam sensitivitas dan akurat.
Oleh karena itu penelitian ini bertujuan untuk mengemhangkan diagnosis
altematif pemariksaan malaria yang lebih sensitif dan akurat. Teknik PCR pada
sampel urin tems dikembangkan sehagai altematif diagnosis malaria. Penelitian
ini dileknkan pada 58 sampel urin yang diambil pada orang yang tlnggal di daerah
endemik malaria dan dipariksa dengan teknik PCR dengan menggunakan primer
ssu rRNA , didapatkan 42 sampel positif dengan sensitivitas 98 % dan spesilisitas
94 %. Uji diagnostik mikroskopik pada sampel darah dan PCR pada sampel untuk
P falclparum didapatkan 18 posltif dengan sensitivitas 94% dan spesifisitas 94%,
sedangkan untuk P. vlvax didapatkan 25 sampel positlf dengan sensitivitas 96%
dan spesifisitas 94% . Teknik PCR dengan sampel urin dapat digunakan sebagai
alat diagnostik malaria untuk menggantikan pemeriksaan mikroskopik darah
karena memilild sensitivitas dan spesifisitas yang tinggi (lebih dari 90% ).
<hr>
<b>Abstract</b><br>
Malaria is an infectious disease which is still causing a public health problem in
many parts of Indonesia. There are many endemic areas where the prevalence of
malaria is high . The diagnosis of malaria is commonly done by clinical
examination and parasite finding at microscopic examination of blood sample.
Microscopic examination is still used as a gold standard for malaria diagnosis,
however this method is less sensitivity and accuracy especially in low parasitemia.
Therefore, it is a need to develop an alternative method which is more sensitive
and accurate fur Malaria diagnosis. PCR method for urine sample is being
developed as an alternative diagnosis for Malaria. A total of 58 individuals living
in malaria endemic areas participated in blood and urine collections. The
presence of malaria parasites in blood samples were detected by microscopic
examination whereas the DNA of mrdarial parasites, P. falciparum and P. vivax,
in urine samples were detected by PCR method using ssu rRNA primers. Positive
results of both malarial parasites were found in 42 samples with 98% sensitivity
and 94 % specificity. Diagnostic test of microscopic examination of blood
samples and PCR of urine samples showed that 18 samples were P. falciparum
positive with 94% sensitivity and 94% specificity whereas 25 samples were
positive for P. vivax with 96% sensitivity and 94% specificity This study revealed
that PCR method can be used as an alternative diagnostic tool for malaria because
it has high sensitivity and specificity (more than 90 %).</description><identifier>https://lib.ui.ac.id/detail?id=20341278</identifier><recordID>20341278</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Bachelors |
| author |
Jonno Berty Bradly Bernadus, author Add author: Heri Wibowo, supervisor Add author: Hendri Astuty, supervisor Add author: Taniawati Supali, examiner Add author: J. S. B. Tuda, examiner |
| title |
Diagnosis malaria pada sampel urin dengan teknik polymerase chain reaction |
| publishDate |
2009 |
| topic |
Malaria --Diagnosis |
| url |
https://lib.ui.ac.id/detail?id=20341278 |
| contents |
<b>ABSTRAK</b><br>
Malaria merupakan penyakit yang masih menimbulkan masalab kesehatan
Masyarakat Indonesia. Prevalensi malaria di beberapa daerah cukup tinggi dan
menjadikan daerah tersebut endemik malaria. Diagnosis malaria ditegakkan
melalui pemariksaan gejala ktJnis dan penemuan parasit pada pemariksaan darah
seoara mikroskopik. Pemariksaan mikroskopik masih merupakan "Gold
Standard", tetapi masih terdapat beberapa kendala dalam sensitivitas dan akurat.
Oleh karena itu penelitian ini bertujuan untuk mengemhangkan diagnosis
altematif pemariksaan malaria yang lebih sensitif dan akurat. Teknik PCR pada
sampel urin tems dikembangkan sehagai altematif diagnosis malaria. Penelitian
ini dileknkan pada 58 sampel urin yang diambil pada orang yang tlnggal di daerah
endemik malaria dan dipariksa dengan teknik PCR dengan menggunakan primer
ssu rRNA , didapatkan 42 sampel positif dengan sensitivitas 98 % dan spesilisitas
94 %. Uji diagnostik mikroskopik pada sampel darah dan PCR pada sampel untuk
P falclparum didapatkan 18 posltif dengan sensitivitas 94% dan spesifisitas 94%,
sedangkan untuk P. vlvax didapatkan 25 sampel positlf dengan sensitivitas 96%
dan spesifisitas 94% . Teknik PCR dengan sampel urin dapat digunakan sebagai
alat diagnostik malaria untuk menggantikan pemeriksaan mikroskopik darah
karena memilild sensitivitas dan spesifisitas yang tinggi (lebih dari 90% ).
<hr>
<b>Abstract</b><br>
Malaria is an infectious disease which is still causing a public health problem in
many parts of Indonesia. There are many endemic areas where the prevalence of
malaria is high . The diagnosis of malaria is commonly done by clinical
examination and parasite finding at microscopic examination of blood sample.
Microscopic examination is still used as a gold standard for malaria diagnosis,
however this method is less sensitivity and accuracy especially in low parasitemia.
Therefore, it is a need to develop an alternative method which is more sensitive
and accurate fur Malaria diagnosis. PCR method for urine sample is being
developed as an alternative diagnosis for Malaria. A total of 58 individuals living
in malaria endemic areas participated in blood and urine collections. The
presence of malaria parasites in blood samples were detected by microscopic
examination whereas the DNA of mrdarial parasites, P. falciparum and P. vivax,
in urine samples were detected by PCR method using ssu rRNA primers. Positive
results of both malarial parasites were found in 42 samples with 98% sensitivity
and 94 % specificity. Diagnostic test of microscopic examination of blood
samples and PCR of urine samples showed that 18 samples were P. falciparum
positive with 94% sensitivity and 94% specificity whereas 25 samples were
positive for P. vivax with 96% sensitivity and 94% specificity This study revealed
that PCR method can be used as an alternative diagnostic tool for malaria because
it has high sensitivity and specificity (more than 90 %). |
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