Analisis hubungan konsumsi glukosa, aktivitas spesifik dan pola isozim Lactate Dehydrogenase (LDH) pada hati tikus yang diinduksi hipoksia sistemik kronik = Analysis of the relationship of glucose consumption, specific activities and iodozyme patterns of Lactate Dehydrogenase (LDH) in rat liver tissue during induced ebronic systemic hypoxia
| Main Authors: | Agus Evendi, author, Add author: Sri Widia Jusman, supervisor, Add author: Dwirini Retno Gunarti, examiner, Add author: Dwirini Retno Gunarti, supervisor, Add author: Ninik Mudjihartini, examiner, Add author: Dwi Anita Suryandari, examiner, Add author: Freisleben, Hans-Joachim, examiner |
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| Format: | Masters Bachelors |
| Terbitan: |
, 2010
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| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20341179 |
Daftar Isi:
- <b>ABSTRAK</b><br> Penelitian ini bertujuan untuk mempeiajari konsumsi glukosa. aktivitas spesifik LDH dan pola isozim LDH pada kondisi hipoksia sistemik kronik. Penelitian dilakukan terhadap hati tikus yang diinduksi hipoksia sistemik kronik 1, 3. 7 dan 14 hari. Konsumsi glukosa diukur dengan metode enzimatik Trlnder. Aktivitas spesiflk LDH diukur dengan metode German Society of Clinical Chemistry (DGKC). Poia isozim LDH dianaJisis dengan eJektroforesis Titan Gel. Hasil peneHtian menunjukkan bahwa konsumsi g!ukosa pada hipoksia sistemik kronik cenderung rneningkat walaupun tidak. berbeda dengan kontrol. Aktivitas spesIfik LDH ditemukan paling tinggi pada hipoksia 3 hari sedangkan pola elektroforesis isozim LDH menunjukkan perbeda.an pada hari ke-3 dan ke-7 hipoksia dengan kontroL Tidak terdapat hubungan antara konsumsi glukosa dengan aktivitas spesifik LDH. <hr> <b>ABSTRACT</b><br> The aim of this study was to observe glucose consumption, specific activity of LDH and electrophoretic LDH isoenzyme patterns on systemic chronic hypoxia. The study was carried out with Jiver tissue of rats exposed to systemic hypoxia for 3, 7 and 14 days. Glucose consumption was measured by Trinder method. The specific activity ofLDH was performed using Gcnnan Society of Clinical Chemistry (DGKC) method, while LDH isoenzyme patterns were analyzed using Titcm Gel electrophoresis, Results indicated that glucose consumption showed tendencies to increase compared to control group, although the difference were not sigr.itlcant. The specific activity of LDH was highest on day 3 of the hypoxic group. Electrophoretic patterns of LDH isoenzyme showed differences on day 3 and day 7 of hypoxia with controL It is concluded that glucose consumption is not related to specific activity of LDH.