3 variasi GEN E, NSI, NS3 dari Virus Dengue Type 3 = Variety of E, NSI, NS3 gene of Type3 Dengue Virus
| Main Authors: | Rayhana, author, Add author: Tjahjani Mirawati Sudiro, supervisor, Add author: Agus Sjahrurachman, supervisor, Add author: Andriansjah, examiner, Add author: Mohamad Sadikin, examiner, Add author: Purnomo Soeharso, examiner |
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| Format: | Masters Bachelors |
| Terbitan: |
, 2009
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| Subjects: | |
| Online Access: |
https://lib.ui.ac.id/detail?id=20340191 |
Daftar Isi:
- <b>ABSTRAK</b><br> Demam berdarah dengue merupakan salah satu penyakit wabah di didunia yang telah merenggut banyak nyawa. Pada tahrm 2004 terjadi wabah demam berdarah dengue di Jakarta dengan lebih dari 10.000 kasus dengan angka kematian dilaporkan sebanyak 603 orang dengan kasus infeksi serotipe virus dengue terbanyak adalah serotipe 3. Penelitian ini menggunakan metode PCR. Pada penelitian ini dilakukan analisis variasi genetik gen E, NSI, dan NS3 dari virus dengue tipe 3 yang diisolasi dari pasien dengan manifestasi klinis yang berbeda. Strain DS2207 (DD), DS-4607 (DBD), DSA0206 (DSS) yang diisolasi dari kasus dengue 2006-2007 dan dibandingkan clengan 12 strain yang berasal dari Indonesia, Thailand, Tahiti, Venezuela, Malaysia. Homologi nuldeotida gen E berkisar antara 92,4 - 100%, sedangl-can asam amino E 97 - l00%. Homologi nukleotida gen NSI berkisar antara 93,4 - 100%, sedangkan asam amino NS1 97,2 - 100% Homologi nukleotida gen NS3 berkisar antara 92,8 - 99,5%, sedangkan asam amino NS3 98,2 - 99,'7%. Terdapat variasi pada ketiga gen, tapi belum ditemukan adanya perbedaan spesifik dari manifestasi klinis yang ditimbulkan. <hr> <b>Abstract</b><br> Dengue haemorrhagic fever is one of the epidemical disease that widely spread and has taken many lives. In the year of 2004, the epidemic of dengue haemorrhagic fever occurred in Jakarta with more than 10,000 number of cases, and 603 reported to death, where serotype 3 showed as the most dengue virus serotype infection cases. This research used the PCR (polymerase chain reaction) method, along with an analysis in genetical variety of E, NS1, and NS3 Gene of isolated Type 3 Dengue Virus (from the patient with any different clinical symptom). Strain of DS2207 (Dengue fever), DS4607 (Dengue haemorrhagic fever), DSA0206 (Dengue shock syndrome) has been isolated of the 2006-2007 dengue cases, and compared with 12 strain from Indonesia, Thailand, Tahiti, Venezuela, and Malaysia. Nucleotide homology of the E gene is somewhere around 92,4% up to l00%, with the E amino acid reached 97% up to 100%. Nucleotide homology of the NSI gene is somewhere around 93,4% up to l00%, with the NSI amino acid reached 97,2% up to l00%. Nucleotide homology of the NS3 gene is somewhere around 92,8% up to 99,5%, with the NS3 amino acid reached 98,2% up to 99,7%. There were also variety of those three genes, without any specific differences among different manifestations.