Perbaikan neuron hipokampus pascahipoksia serebri dengan penggunaan ekstrak air akar tanaman akar kucing = The improvement of hippocampus neuron post serebral hypoxia with aqueous extract of acalypha indica Linn Root
| Main Authors: | Lolo Suswanti, Add author: Nurhadi Ibrahim, supervisor, Add author: Erni Hernawati Purwaningsih, supervisor, Add author: Sri Redjeki, examiner, Add author: Sri Widia Jusman, examiner, Add author: Franciscus D. Suyatna, examiner |
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| Format: | Masters Bachelors |
| Terbitan: |
, 2009
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| Online Access: |
https://lib.ui.ac.id/detail?id=20339828 |
Daftar Isi:
- [<b>ABSTRAK</b><br> Latar belakang : Acalypha indica Linn. merupakan tanaman yang mudah didapat., tumbuh sepanjang tahun dan bisa ditemukan di kebun, halaman rumah maupun tempat-tempat pembuangan sampah. Semua bagian dari tanaman ini bisa digunakan dalarn pengobatan tradisional untuk menangani berbagai masalah kesehatan. Pada penelitian sebeiumnya didapatkan data bahwa ekstrak Acalypha indica Linn. terbukti memiliki efek sebagai neuroproteksi dan neuroterapi pada neuromuscular junclion jaringan katak dan mampu menghambat efek neurotoksik pada jaringan katak. Oleh karena itu, ekstrak air akar Acalypha indica Linn. bisa mernpengaruhi perbaikan neuron hipokampus pascahipoksia serebri. Tujuan : untuk menganalisa kerusakan sel neuron hipokampus pascahipoksia serebri setelah pemberian ekstrak air akar Acalypha indica Linn. Metode penelitian : 30 ekor tikus Sprague Dawley terbagi secara acak dalam enam kelompok. Kelompok hipoksia, tikus dimasukkan ke dalam sungkup hipoksia yang mengandung gas campuran (02 10 % dan nitrogen 90 %) selama tujuh hari, untuk kelompok reoksigenasi tikus dibiarkan menghirup udara bebas setelah perlakuan hipoksia. Tiga kelompok terapi lainnya setelah perlakuan hipoksia tikus kemudian dilanjutkan dengan pemberian ekstrak air akar Acalypha indica Linn pada dosis 300, 400 dan 500 mg/kg BB selama tujuh hari. Kriteria penilaian yang digunakan dalam studi ini adalah jumlah sel rusak neuron hipokampus dengan mengamati : bentuk sel, ada tidaknya kodensasi krornatin, piknotik dan rasio sitoplasma dengan inti setelah pewarnaan hemaktosilin eosin. Hasil : ekstrak air akar Acalypha indica Linn. pada dosis 400 dan 500 mg/kg BB secara signifikan mampu mernperbaiki kerusakan sei neuron (efek neuroterapi) hipokampus pascahipoksia serebral (p = 0,01). Kesimpulan : ekstrak air akar Acalypha indica Linn memiliki efek neuroterapi pada dosis 400 dan 500 mg/kg BB. <hr> <b>ABSTRACT</b><br> Background : Acalypha indica Linn is a common herb which can easily be found elsewhere in Indonesia, All the parts of plants are used in various traditional therapy for some diseases. Previous studies showed that Acalypha indica Linn extracts have neuro-protective and neuro-therapy effects on neuromuscular junction and inhibit neurotoxin on isolated frog tissues. Thus, administration of aqueous extracts of Acalyjpha. indica Linn root was assumed to improve hippocampus neuron injury after cerebral hypoxia. Objective : To investigate the effects of aqueous extracts of Acalypha indica Linn root on hippocampus neuron injury after cerebral hypoxia in the rat. Methods : Thirty male Sprague Dawley with 200-250 gr Body weight of rats were divided into six groups randomly. The rats were housed in hypoxic chamber containing gas mixture of I0 % O2 and 90 % N2 for seven days, followed by administration of 300, 400 and 500 mg/kg BW aqueous extracts of A. indica Linn root for seven days.. The other group was exposed to room air after hypoxia. The parameters measured were hippocampal cell damage ie : the quantity and type of hippocampal cell, chromatin condensation, pycnotic and cytoplasm nucleus ratio using hematoxyline eosin staining. Result : The aqueous extracts of A. indica Linn root of 400 and 500 mg/kg BW improve hippocampus neuron injury (neurothcrapy effect) alter cerebral hypoxia significantly (p = 0,0l). Conclusion : The aqueous extracts of A. indica Linn roots have neurotherapy effects on hippocampal neuron after cerebral hypoxia of 400 and 500 mg/kg BB. , Background : Acalypha indica Linn is a common herb which can easily be found elsewhere in Indonesia, All the parts of plants are used in various traditional therapy for some diseases. Previous studies showed that Acalypha indica Linn extracts have neuro-protective and neuro-therapy effects on neuromuscular junction and inhibit neurotoxin on isolated frog tissues. Thus, administration of aqueous extracts of Acalyjpha. indica Linn root was assumed to improve hippocampus neuron injury after cerebral hypoxia. Objective : To investigate the effects of aqueous extracts of Acalypha indica Linn root on hippocampus neuron injury after cerebral hypoxia in the rat. Methods : Thirty male Sprague Dawley with 200-250 gr Body weight of rats were divided into six groups randomly. The rats were housed in hypoxic chamber containing gas mixture of I0 % O2 and 90 % N2 for seven days, followed by administration of 300, 400 and 500 mg/kg BW aqueous extracts of A. indica Linn root for seven days.. The other group was exposed to room air after hypoxia. The parameters measured were hippocampal cell damage ie : the quantity and type of hippocampal cell, chromatin condensation, pycnotic and cytoplasm nucleus ratio using hematoxyline eosin staining. Result : The aqueous extracts of A. indica Linn root of 400 and 500 mg/kg BW improve hippocampus neuron injury (neurothcrapy effect) alter cerebral hypoxia significantly (p = 0,0l). Conclusion : The aqueous extracts of A. indica Linn roots have neurotherapy effects on hippocampal neuron after cerebral hypoxia of 400 and 500 mg/kg BB. ]