SOMATIC EMBRYOGENESIS IN MUNG BEANS (Vigna radiata L.)

Main Authors: Bhor Abhishek K., Cholke Pravin B., Shete Akshay M.
Format: Article eJournal
Terbitan: , 2019
Subjects:
Online Access: https://zenodo.org/record/4091529
Daftar Isi:
  • Somatic embryos were obtained from hypocotyl region of Vigna radiata seeds. MS medium supplemented with various growth regulators was used for callus induction. The callus is developed from hypocotyl region. Medium composition is MS+ 5 mg/L NAA+ 2mg/L 2, 4 D. After callus development this callus transferred into liquid MS medium with Proline, 2, 4 D. After application of liquid medium embryos are developed & this embryo transfer into another MS liquid medium with BAP and L- Proline and the developmental stages of embryo under observed the Research microscope.