Figure-1e
| Main Author: | Pawan kumar |
|---|---|
| Format: | info Image eJournal |
| Terbitan: |
, 2020
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/4015071 |
Daftar Isi:
- Higher electrophoretic mobility DNA (HmDNA) in NaOH-denatured pUC19 is single-stranded and circular in nature. pUC19 plasmid was denatured using 0.5 N NaOH to produce HmDNA. After ~5 min, NaOH was neutralized using 0.5 M HCl, and DNA was incubated with exonuclease I (Exo I) and alkaline phosphatase (AP), which would digest single-stranded linear DNA. Neither of native pUC19 or HmDNA was degraded by Exo I and AP (lane 7). Experiment was repeated two times (i, ii). Lane 1, 1 kb ladder; Lane 2, pUC19; Lane 3, pUC19 + 0.5 N NaOH; Lane 4, pUC19 + 0.5 N NaOH + 0.5 M HCl; Lane 5, pUC19 + ‘Exo I + AP’; Lane 6, pUC19 + 0.5 N NaOH + ‘Exo I + AP’; Lane 7, pUC19+ 0.5 N NaOH + 0.5 M HCl + ‘Exo I + AP’.