Effects of CB2 Receptor Agonist JWH133 on Aβ42-Induced Hippocampal Neurons
| Main Author: | Zhao, Jingfu |
|---|---|
| Format: | Article eJournal |
| Bahasa: | eng |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3980103 |
Daftar Isi:
- Objective To observe the effects of Cannabinoid type 2 receptor (CB2R) on Aβ-induced hippocampal neuron. Methods Hippocampal neurons were pre-treated with 10 μM JWH133 and then treated 100 nM Aβ42 oligomer for 7 day, the mRNA of Bcl-2 and Bax was measured by qRT-PCR, and the mitochondrial membrane potential and the production of ROS were measured by Flow Cytometry. Result Aβ42 oligomer significantly decreased the ratio of Bcl-2/Bax, and mitochondrial membrane potential, and increased the production of ROS. However, the pre-treatment JWH133 inhibited Aβ42-induced the ratio of Bcl-2/Bax decrease, and increased the mitochondrial membrane potential, decreased the production of ROS. Conclusion Activation of CB2R could inhibit the effects of Aβ42 on hippocampal neurons.Read Complete Article at ijSciences: V92020062353 AND DOI: http://dx.doi.org/10.18483/ijSci.2353