Dataset related to the article "Cyclophilin A in Arrhythmogenic Cardiomyopathy Cardiac Remodeling"
| Main Authors: | Rurali Erica, Pilato Chiara Assunta, Perrucci Gianluca Lorenzo, Scopece Alessandro, Stadiotti Ilaria, Moschetta Donato, Casella Michela, Cogliati Elisa, Sommariva Elena, Pompilio Giulio, Nigro Patrizia |
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| Format: | info dataset eJournal |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3701241 |
Daftar Isi:
- This record contains raw data related to the article "Cyclophilin A in Arrhythmogenic Cardiomyopathy Cardiac Remodeling" Abstract: Arrhythmogenic cardiomyopathy (ACM) is a genetic disorder characterized by the progressive substitution of functional myocardium with noncontractile fibro-fatty tissue contributing to ventricular arrhythmias and sudden cardiac death. Cyclophilin A (CyPA) is a ubiquitous protein involved in several pathological mechanisms, which also characterize ACM (i.e., fibrosis, inflammation, and adipogenesis). Nevertheless, the involvement of CyPA in ACM cardiac remodeling has not been investigated yet. Thus, we first evaluated CyPA expression levels in the right ventricle (RV) tissue specimens obtained from ACM patients and healthy controls (HC) by immunohistochemistry. Then, we took advantage of ACM- and HC-derived cardiac mesenchymal stromal cells (C-MSC) to assess CyPA modulation during adipogenic differentiation. Interestingly, CyPA was more expressed in the RV sections obtained from ACM vs. HC subjects and positively correlated with the adipose replacement extent. Moreover, CyPA was upregulated at early stages of C-MSC adipogenic differentiation and was secreted at higher level over time in ACM- derived C-MSC. Our study provides novel ex vivo and in vitro information on CyPA expression in ACM remodeling paving the way for future C-MSC-based mechanistic and therapeutic investigations.
- This study was supported by Ministero della Salute (RC 2016-2017 to Centro Cardiologico Monzino—IRCCS).