Daftar Isi:
- The cerebral neocortex is the most newly-evolved region of the brain. In humans it has undergone dramatic progenitor expansion, subtype specification, and network formation. Human induced pluripotent stem cell (iPSC)-derived neural progenitors have shown an in vivo -like ability to self-organise & promote cerebral cortical lineage determination under minimal growth factor conditions (Shi et al., 2012a; Renner et al., 2017). However, although the cortex’s laminated organisation represents a great functional significance in vivo , neocortical networks are rarely modelled in vitro using 3D cell culture, or omit key neural subtypes (Kirwan et al 2015). Despite recent developments in tissue modelling, e.g. organoids (Kelava & Lancaster, 2016), 3D models exhibit high variability – impedingexplorationofnetworktopography.