NEUROPROTECTIVE ACTIVITY OF HESPERIDIN AGAINST MPTP (1-METHYL-4-PHENYL-1, 2, 3, 6-TETRAHYDROPYRIDINE) INDUCED NEUROTOXICITY: IN VITRO APPROACHES
| Main Author: | G. Devala Rao, Hanumanthu Penchalaiah* S Praveen Begum |
|---|---|
| Format: | Article eJournal |
| Terbitan: |
, 2018
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| Online Access: |
https://zenodo.org/record/3593564 |
Daftar Isi:
- Aim: In the present study, we examined the neuroprotective activity of hesperidin on the accumulation of neuronal oxidative stress induced by MPTP by in vitro. Method: After decapitation, healthy rat brain was removed rapidly from the skull and rinsed with cold artificial cerebrospinal fluid (ACSF) which has been equilibrated with 95% O2/5 % CO2 gas mixture. Group I brain slices was incubated in ACSF serve as normal, Group II brain Slices was incubated in ACSF and DMSO (10%) serve as disease, Group III brain was incubated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (1 ng/ml) Group IV brain was incubated in MPTP with Bromocriptine (10 μg/ml) as a standard, Group V brain was incubated in MPTP with hesperidin (10 μg/ml) Group VI brain was incubated in MPTP with hesperidin (20 μg/ml). After 1hr of incubation brain slices were homogenized in PBS buffer, pH 7.4 and supernatant subjected estimation of for protein content, lipid peroxidase and reduced glutathione. Results and conclusion: MPTP incubated brain LPO* activity was significantly (p<0.001***) increased and GSH activity decreased (p<0.001***) compared to normal group. Hesperidin incubated group brain LPO* activity was significantly (p<0.05*) decreased and GSH activity was increased (p<0.01**), The research results were concluded that the hesperidin exhibited significant neuroprotectivite effect against MPP+ free radicals due to their antioxidant activity. Keywords: Hesperidin, Cerbrospinal fluid, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), Brain slices, Lipid peroxidase (LPO*),Glutathione (GSH)