Optimization of antibiotic activity of composites of ethanolic extracts of flower of Mangifera indica, Gongronema latifolium leaves and Citrus sinensis peel using the mixture experimental design of the response surface methodology
| Main Authors: | Ta'awu, K. G., Ekanem, M. C., Udofia, P. G. |
|---|---|
| Format: | Article eJournal |
| Bahasa: | eng |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3558833 |
Daftar Isi:
- Abstract— The generation of pathogenic microorgannisms is overwhelming the potency, safety and cost of synthetic antibiotics. The study south insight for the use of plant materials to fight microbes and optimized antibiotic activity of pure, binary and ternary blends of ethanol extracts of flower of Mangifera india, Gongronema latifolium leaves, Citrus sinensis peel on Streptococcus aureus using the Simplex Lattice {3,3} mixture experimental design of the response surface methodology (RSM). Fourteen (14) blends of the plants' parts were produced and tested on the S. aureus. Inhibition zones inhibited by the extract blends ranged between 11-19 mm. Blends C and BC exhibited the highest value of 19 mm. Other blends equally exhibited some inhibition effects on the growth of the test microorganism, however, decreasing in values as their proportions in the blends carried. ANOVA on the data revealed that the model of the experiment was significant (p<0.05; R2=0.8350), the pure; A, B, C, and the ternary blends, ABC, were significant in the model (p<0.05). Although other blends were n ot statistically significant (p>0.05), the graphic and the equation indicated their positive contributions to the model. The model showed overall mean inhibition zone of 14.11 mm compared to 22.5 mm observed in Levofloxacin on the test organism. The study showed that ethanolic extracts of the plants' parts could provide the basis for engineering and synthesis of potent antibiotics.