Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation
| Main Authors: | Hung-Jen Chien, Tsai-Chen Chiang, Shih-Jung Peng, Mei-Hsin Chung, Ya-Hsien Chou, Chih-Yuan Lee, Yung-Ming Jeng, Yu-Wen Tien, Shiue-Cheng Tang |
|---|---|
| Format: | info Video eJournal |
| Terbitan: |
, 2019
|
| Online Access: |
https://zenodo.org/record/2654432 |
Daftar Isi:
- Supplemental Video Legends Supplemental Video 1 (related to Figure 2) Human pancreatic substance P+ sensory (afferent) nerves. Left (still image): projection of pancreatic innervation at the acinar-islet boundary. Blue: glucagon (islet). Green: neuroendocrine marker PGP9.5 (nerve and islet). Red: substance P (SP, sensory nerve). Sensory nerves appear as yellow fibers (overlap of red SP and green PGP9.5 signals). Broken arrow: enlarged area (right panel). Right: in-depth recording of peri-ductal sensory nerves. Sensory nerves (yellow) are around the islet but not penetrating into the core. White: nuclei. Note that a portion of islet cells are SP dim. Supplemental Video 2 (related to Figure 2 and Supporting Fig. 1) Mapping and detection of substance P+ sensory neuron in duct lesion formation. Left (still image): tissue map of human pancreas distal to ductal adenocarcinoma. Acinar atrophy is apparent in this condition. Markers: nuclei, white; PGP9.5, green; substance P (SP), red. Broken arrow: intra-pancreatic ganglion (right panel). Right: in-depth recording of SP+ sensory neuron in ganglion. The nucleus (00:04) and the SP+ varicosities are prominently seen. The latter contacts the surrounding SP- neurons. Supplemental Video 3 (related to Figure 3) Mouse pancreatic substance P+ sensory (afferent) nerves. Left (still image): projection of pancreatic sensory innervation. In mice, the SP+ sensory nerves follow the arteriole to the islet mantle and penetrating into the core (enlarged in right panel). Markers: blood vessels, blue; PGP9.5, green; substance P (SP), red. Right: in-depth recording of sensory innervation of islet. Unlike the human pancreatic sensory innervation, the mouse SP+ sensory nerves (varicosities) are inside the islet and associate with the peri-islet ganglion. White: nuclear staining and tissue autofluorescence.