Features prevalence of polymorphism lle50Val gene IL4RA in patients with bronchial asthma and healthy children school age Vinnichina
| Main Authors: | Dudnyk, V. M., Kutsak, O. V. |
|---|---|
| Format: | Article eJournal |
| Terbitan: |
, 2017
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| Online Access: |
https://zenodo.org/record/1206402 |
Daftar Isi:
- Dudnyk V. M., Kutsak O. V. Features prevalence of polymorphism lle50Val gene IL4RA in patients with bronchial asthma and healthy children school age Vinnichina. Journal of Education, Health and Sport. 2017;7(5):934-943. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1206402 http://ojs.ukw.edu.pl/index.php/johs/article/view/5382 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2017; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.05.2017. Revised: 23.05.2017. Accepted: 31.05.2017. UDK: 616.248-053.2:575.113(477.44) FEATURES PREVALENCE OF POLYMORPHISM lle50Val GENE IL4RA IN PATIENTS WITH BRONCHIAL ASTHMA AND HEALTHY CHILDREN SCHOOL AGE VINNICHINA V. M. Dudnyk, MD, Professor, O. V. Kutsak, postgraduate Vinnitsa National Pirogov Memorial Medical University, Vinnitsa, Ukraine Department of Pediatrics No2 Abstract Today, the mechanism of development of bronchial asthma (BA) is interpreted from the molecular-genetic point of view, taking into account the polymorphism of genes that affect the formation and clinical manifestations of it. Studying the genetic aspects of bronchial asthma in children is aimed at studying various associations of polymorphisms of genes of interleukin ligands and their receptors. The aim - to evaluate the prevalence of genotypes and alleles for the gene polymorphism lle50Val IL4RA in patients with asthma and healthy school children Vinnitsa region. Materials and methods. We examined 75 children of school age, patients with atopic bronchial asthma and 25 practically healthy children aged 6 to 18 years living in Vinnitsa region. The genotyping of IL4RA, SNP polymorphism lle50Val was carried out using a polymerase chain reaction (PCR). The odds ratio was calculated to determine the risk of developing asthma in carriers of various alleles and genotypes of the IL4RA gene. Results and discussion. Homozygous genotype A / A Vinnichiny among school children suffering from asthma occurs in 4.34 times (p <0,05) more often and genotype G / G - 4.2 times (p <0,05) less compared to practically healthy children with prevalence 1.6 times allele a carriers (p <0,05), respectively, carrier genotype a / a and a allele is associated with the development of asthma and is a marker of increased risk of disease, and the presence of the genotype G / G and allele G - "unfavorable" to the development of bronchial asthma. The distribution of the frequency of genotypes for the Ile50Val polymorphism of the IL4RA gene is independent of sex and indicates a collision lshennya heterozygous genotype A / G patients among boys in 1.84 times, and among girls - by 1.3 times compared with native homozygous genotype A / A (p <0,001) and, respectively, 8.74 and 9 times - compared with a mutant genotype G / G (p <0.001). Conclusions. Established that the carrier genotype A / A and A allele asootsiyuyetsya with the development of asthma and the presence of the genotype G / G and G alleles can be considered a factor that affects the "unfavorable" development of asthma in children of school age. Key words: bronchial asthma, children, polymorphism of gene IL4RA.