Identification of Single Nucleotide Polymorphism on Growth Hormone Gene in Aceh Cattle
| Main Authors: | Sari, E M, Noor, R R, Sumantri, C, Yunus, M, L, Han J, Muladno, . |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Animal Science, Bogor Agricultural University
, 2013
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/6841 http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/6841/5251 http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/6841/5263 |
Daftar Isi:
- This research was aimed to identify the changes of nucleotide (Single Nucleotide Polymorphism) growth hormone gene in the population of Aceh cattle. There were 44 samples of DNA sequenced, and a few samples from Gen Bank (M57764). Based on the analysis using MEGA program, it was identified one new mutation on exon five on 2230 bp in which C nucleotide turned into T nucleotide, and this was called Silent Mutation (Leusine-Leusine/ CTC-CTT). The frequency of Single Nucleotide Polymorphism (SNP) genotype on 2230 bp (C/T) was CC (0.36), TT (0.14) and CT (0.50). The genotype TT was not possessed by Aceh cattle from Saree, but possessed by those from Banda Aceh and Indrapuri. Chi-square test showed not significant differences in allele frequencies for three population. The frequency of genotype SNP on 2291 bp (A/C) was AC (0.11) and CC (0.89). The frequency of allele C was higher than allele A and T.