Daftar Isi: Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure

Main Authors:	Herawati, Margaretha; Master Program in Biomedical Sciences, Faculty of Medicine University of Indonesia Department of Biochemistry, Faculty of Dentistry, Universitas Prof. Dr. Moestopo (Beragama), Jakarta, Wardaya, .; Indonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, Jakarta, Mulyawan, Wawan; Indonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, Jakarta, Farhan, Fanny Septiani; Department of Physiology, Faculty of Medicine Muhammadiyah University, Jakarta, Ferdinal, Frans; Department of Biochemistry and Molecular Biology, Faculty of Medicine Tarumanagara University, Jakarta, Jusman, Sri Widia A.; Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta, Sadikin, Mohamad; Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta
Format:	Article info application/pdf eJournal
Bahasa:	eng
Terbitan:	Bogor Agricultural University, Indonesia , 2018
Subjects:	intermittent hypobaric hypoxia HIF-1a Mb
Online Access:	http://journal.ipb.ac.id/index.php/hayati/article/view/20807 http://journal.ipb.ac.id/index.php/hayati/article/view/20807/14253

Daftar Isi:

The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.