Effect of Probiotic Bacillus megaterium PTB 1.4 on the Population of Intestinal Microflora, Digestive Enzyme Activity and the Growth of Catfish (Clarias sp.)
| Main Authors: | Afrilasari, Wahyu; Study Program of Microbiology, Department of Biology, Faculty of Mathematic and Natural Science, Bogor Agricultural University, IPB Dramaga Campus, Bogor, West Java, Widanarni, .; Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University, IPB Dramaga Campus, Bogor, West Java, Meryandini, Anja; Department of Biology, Faculty of Mathematic and Natural Science, Bogor Agricultural University, IPB Dramaga Campus, Bogor, West Java |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Bogor Agricultural University, Indonesia
, 2018
|
| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/hayati/article/view/20730 http://journal.ipb.ac.id/index.php/hayati/article/view/20730/14226 |
Daftar Isi:
- This study aimed to analyze the effect of Bacillus megaterium PTB 1.4 on the population of intestinal microflora, digestive enzyme activity, and the growth of catfish. Gnotobiotic and normal fish were used. Treatment using gnotobiotic was divided into gnoto (with feed and 100 μg/mL rifampicin) and gnotoplus (with feed, 100 μg/mL rifampicin, and 1% probiotic); whereas treatment using normal fish was divided into normalplus (with feed and 1% probiotic) and normal (only feed). The amount of bacteria on gastrointestinal tract was measured 30 days after treatments using the total plate count method. The results indicated no significant difference in bacterial growth between gnotobiotic and normal fish. The total amount of probiotic bacteria with normalplus treatment was significantly different with gnotoplus. The activity of protease and amylase enzymes, and specific growth rate in normalplus treatment were significantly higher (p < 0.05) than other treatments. Bacillus megaterium PTB 1.4 increased the activity of digestive enzymes and the growth of catfish.