PENGARUH PEMBERIAN TEPUNG KEDELAI KAYA ISOFLAVON TERHADAP KADAR MALONALDEHID (MDA), AKTIVITAS SUPEROKSIDA DISMUTASE (SOD) TESTIS DAN PROFIL Cu,Zn-SOD TUBULI SEMINIFERI TESTIS TIKUS JANTAN [The effects of isoflavone-riched soybean flour on MDA Level, Sup
| Main Authors: | Astuti, Sussi, Muchtadi, Deddy, Astawan, Made, Purwantara, Bambang, Wresdiyati, Tutik |
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| Format: | Article info application/force-download eJournal |
| Bahasa: | eng |
| Terbitan: |
Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI
, 2009
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jtip/article/view/1958 http://journal.ipb.ac.id/index.php/jtip/article/view/1958/3862 |
Daftar Isi:
- The objectives of this experiment were to evaluate the effects of isoflavone-riched soybean flour with different treatment level of isoflavone on MDA level, SOD activity, and profile of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) spermatogenic cells in the seminiferous tubules of male rat testes. Diet was given as isonitrogen and isocaloric with 10% of dietary protein. Twenty five males of Sprague Dawley rats were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different concentrations. The treatment was done for 2 months. The optimum concentration of isoflavone was 1.5 mg/day and resulted in : decreased level of MDA testes (2.29±0.05 nmol/g), mantained high SOD activity of rat testes (729.4±23.73 U/g), and increased content of Cu,Zn-SOD by immunohistochemical technique on spermatocytes and early spermatids. It was concluded that isoflavone-riched soybean flour diet (1.5 mg/day isoflavone) can prevent the formation of lipid peroxide and improve the antioxidant status of rat testes.