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Partial Sequencing of 16S rRNA Gene of Selected Staphylococcus aureus Isolates and its Antibiotic Resistance

Tersimpan di:
Main Authors: Kusumaningrum, Harsi Dewantari; Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Handayani, Lita; Food Science Study Program, Graduate School, Bogor Agricultural University, Nofrianti, Reni; Food Science Study Program, Graduate School, Bogor Agricultural University
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Faculty of Animal Science, Bogor Agricultural University , 2016
Subjects:
16S rRNA gene sequencing
Staphylococcus aureus
enterotoxin-A
antibiotic
Online Access: http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/11598
http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/11598/9853
http://journal.ipb.ac.id/index.php/mediapeternakan/article/downloadSuppFile/11598/552
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Internet

http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/11598
http://journal.ipb.ac.id/index.php/mediapeternakan/article/view/11598/9853
http://journal.ipb.ac.id/index.php/mediapeternakan/article/downloadSuppFile/11598/552

Lokasi

Koleksi Jurnal Matematika dan Aplikasinya
Gedung Perpustakaan Institut Pertanian Bogor
Institusi Institut Pertanian Bogor
Kota BOGOR
Provinsi JAWA BARAT
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Lihat Juga

  • Partial sequencing of 16s rRNA gene of selected stayphylococcus aureus isolates and its antibiotic resistance
    oleh: ANGGRAENI,A
    Terbitan: (2016)
  • Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence
    oleh: Barus, Tati; Atma Jaya Catholic University of Indonesia, Jakarta 12930, et al.
    Terbitan: (2018)
  • Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle
    oleh: Khasanah, Himmatul; Study Program of Animal Production and Technology, Faculty of Animal Science, Graduate School, Bogor Agricultural University, et al.
    Terbitan: (2016)
  • Three isolates among 138 sponge-associated bacteria were isolated from Waigeo Island, Raja Ampat West Papua Province, Indonesia, have been shown protease inhibitory activity against subtilisin (serine protease), thermolysin (metalloprotease), and crude extract from pathogenic bacteria (Eschericia coli enteropathogenic/EPEC K.1.1, Staphylococcus aureus, and Pseudomonas aeruginosa). Those three isolates were designated as sponge associated bacteria SAB S-12, SAB S-21, and SAB S-17. A simple casein and Sea Water Complete (SWC) double layer agar method was used to screen the bacteria against pathogenic bacteria producing protease, i.e. EPEC K.1.1, S. aureus, and P. aeruginosa. Among them, SAB S-12 isolate showed no inhibitory zone indicated. The isolate had the highest inhibitory activity against subtilisin and crude extract enzyme of pathogenic bacteria, the inhibitory activity was 91.6 and 98.9%, respectively. In addition, the SAB S-21 isolate had the highest inhibitory activity against thermolysin, it was 70.4%. The optimum pH and temperature for protease inhibition of the three isolates was at pH 7.0-8.0 and 40-50 oC respectively. Based on 16S rRNA gene sequence, the closest related with SAB S-12, SAB-17, and SAB-21 isolates was Providencia sp. (92% identity), Paracoccus sp. (86% identity), and Bacillus sp. (% identity), respectively
    oleh: WAHYUDI, ARIS TRI; Department of Biology, Bogor Agricultural University, et al.
    Terbitan: (2011)
  • Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1)
    oleh: NAWANGSIH, ABDJAD ASIH; Bogor Agricultural University, et al.
    Terbitan: (2011)

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