Clonal Propagation of Broccoli, Brassica oleracea L.var. italica through In Vitro Shoot Multiplication
| Main Authors: | Sri Nanan Widiyanto; Department of Biology, Faculty of Mathematics and Natural Sciences Institut Teknologi Bandung, Dwi Erytrina; Department of Biology, Faculty of Mathematics and Natural Sciences Institut Teknologi Bandung |
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| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Institut Teknologi Bandung
, 2009
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| Subjects: | |
| Online Access: |
http://journal.fmipa.itb.ac.id/jms/article/view/80 |
Daftar Isi:
- Shoot-tips of broccoli, Brassica oleracea var. italica cv. Green Valiant, were used as explant source in in vitro clonal propagation. The objective of this research is to examine the influence of growth regulators on axillary shoot induction and root formation. Shoot-tips were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog. Benzyl adenine (BA) and kinetin (KIN) at 0.10-10.0 μM were used either singly or in combination with naphthalene acetic acid (NAA) at 0.01-10.0 μM to induce shoot multiplication. For inducing root formation, NAA, indole acetic acid (IAA) or indole butyric acid (IBA) at 0.10-10.0 μM were applied. Experimental results showed that the addition of BA at 10.0 μM induced axillary shoot proliferation respectively. The addition of KIN induced less shoot multiplication than that of BA. During a four-week period of subculture, a single shoot-tip of broccoli produced 16-20 shoots on medium with the addition of 10.0 μM BA. Elongated shoots were mostly rooted on medium containing 1.0 μM NAA. Plantlets were successfully transferred to a soil-sand mixture medium (1:1) and grown naturally in the green house.