Figure 1 from: Guimarães-Cestaro L, Serrão JE, Message D, Martins MF, Teixeira EW (2016) Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. Journal of Hymenoptera Research 49: 43-50. https://doi.org/10.3897/JHR.49.7061
| Main Authors: | Guimarães-Cestaro, Lubiane, Serrão, José Eduardo, Message, Dejair, Martins, Marta Fonseca, Teixeira, Érica Weinstein |
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| Format: | info Image Journal |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/975339 |
Daftar Isi:
- Figure 1 - Agarose gel (2%) stained with SYBR® Safe of the monoplex and multiplex PCR products referring to the detection thresholds of the pathogens in honey bee products. M: 100 bp marker. 1 2.5 spores/g of pollen for Nosema ceranae 2 15 spores/g of pollen for Ascosphaera apis 3 0.8 spore/g of pollen for Paenibacillus larvae 4 multiplex PCR for Nosema ceranae (2.5 spores/g of pollen), Ascosphaera apis (15 spores/g of pollen) and Paenibacillus larvae (0.8 spore/g of pollen) 5 multiplex positive controls for Nosema ceranae (218 bp), Nosema apis (321 bp), Ascosphaera apis (485 bp) and Paenibacillus larvae (700 bp) 6 negative control. M: 100 bp marker 7 1.25 spores/mL of honey for Nosema ceranae 8 7.5 spores/mL of honey for Ascosphaera apis 9 0.4 spore/mL of honey for Paenibacillus larvae 10 multiplex PCR for Nosema ceranae (1.25 spores/mL of honey), Ascosphaera apis (7.5 spores/mL of honey) and Paenibacillus larvae (0.4 spore/mL of honey) 11 multiplex positive controls for Nosema ceranae (218 bp), Nosema apis (321 bp), Ascosphaera apis (485 bp) and Paenibacillus larvae (700 bp) 12 negative control. M: 100 bp marker 13 5 spores/g of royal jelly for Nosema ceranae 14 30 spores/g of royal jelly for Ascosphaera apis 15 1.6 spores/g of royal jelly for Paenibacillus larvae 16 multiplex PCR for Nosema ceranae (5 spores/g of royal jelly), Ascosphaera apis (30 spores/g of royal jelly) and Paenibacillus larvae (1.6 spores/g of royal jelly) 17 multiplex positive controls for Nosema ceranae (218 bp), Nosema apis (321 bp), Ascosphaera apis (485 bp) and Paenibacillus larvae (700 bp) 18 negative control.