Figure 65 from: Grishin N, Cong Q (2014) A new Hermeuptychia (Lepidoptera, Nymphalidae, Satyrinae) is sympatric and synchronic with H. sosybius in southeast US coastal plains, while another new Hermeuptychia species – not hermes – inhabits south Texas and northeast Mexico. ZooKeys 379: 43-91. https://doi.org/10.3897/zookeys.379.6394
| Main Authors: | Cong, Qian, Grishin, Nick |
|---|---|
| Format: | info Image |
| Terbitan: |
, 2014
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/943131 |
Daftar Isi:
- Figure 65 - DNA ID tags of specimens that are over 100 years old. ID tag #2 is shown as an example. The tag region sequence alignment of the three species: Hermeuptychia sosybius, Hermeuptychia hermybius, and Hermeuptychia intricata is shown in the middle and positions at which sequences differ are highlighted in cyan and boxed. Each of the three species differs from the other two by at least 2 nucleotides, and Hermeuptychia sosybius is different from Hermeuptychia intricata by 4 nucleotides. Forward and reverse primer regions are shaded. DNA of the tag was amplified and sequenced in both forward and reverse directions from two over-100-years-old specimens from the same locality (SC: Clarendon Co.). Forward and reverse sequences traces for the first specimen are shown above the reference sequences and the two traces for the second specimen are shown below. It is clear from the traces that the specimen above (13385G10, Fig. 36) is Hermeuptychia sosybius, (A, T, T, & C at these 4 positions, no contamination seen) and the one below (13385G08, Fig. 33) is Hermeuptychia intricata (G, C, C, & T at these 4 positions and equally unambiguous traces). Nucleotides that identify each specimen are indicated in large letters on yellow background and arrows point to the trace peaks revealing these nucleotides. This strategy was applied to identify 12 very old specimens of three species in a random order and yielded unambiguous identifications for 11 of them. One sample appeared to be contaminated, and the traces showed the presence of several nucleotides in many positions. All 11 DNA-based identifications agreed with genitalic identifications.