ISOLATION, BIOCHEMICAL CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF ARSENIC RESISTANT BACTERIA ISOLATED FROM CONTAMINATED SITES OF UTTAR PRADESH, INDIA
| Main Authors: | Vishvas Hare, Pragati Katiyar, Vinay Singh Baghel |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2017
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/824759 |
Daftar Isi:
- Heavy metals are responsible to create toxicity in environment and these causes serious challenge worldwide. Arsenic is a heavy metal that is non-essential but toxic to all living beings. Arsenic has always been under emerging issue due to its toxicity in living beings including human and animals. Arsenic (As) is a toxic pollutant released into the environment either by natural phenomena or anthropogenic activities. Arsenic is known as carcinogenic element that can harm not only human health but, plant and bacteria as well. Pesticides are the major source for accumulation of As in the agricultural soils. Currently available bioremediation techniques have major disadvantages such as secondary environmental pollution and are less cost effective. Therefore, the present study was aimed to isolate arsenic resistant bacteria from terrestrial environment Bahraich, Uttar Pradesh, India for their potential applications in bioremediation strategies. Total thirty eight isolated arsenic-resistant bacteria were isolated, in which three bacterial isolates BBAU/LP3, BBAU/MMM1, and BBAU/MMM5 were taken for further studies due to their higher resistance ability to As and two isolates BBAU/LP3 and BBAU/MMM1 taken for molecular identification. The 16S rRNA gene sequence of the isolate BBAU/LP3 belongs to the genera Bacillus infantis and BBAU/MMM1 belongs to the genera Bacillus litoralis. The results revealed that our isolates BBAU/LP3 and BBAU/MMM1 encoded arsenite oxidizing gene and arsenate reducing gene respectively that will be useful for the development of efficient bioremediation strategies in the detoxification of arsenic from polluted environments.