Confined Brownian motion analysis of 11 B.Subtillis spores trapped within a 16 waveguide optical trap
| Main Author: | Loozen, Gyllion |
|---|---|
| Format: | info Lainnya Journal |
| Terbitan: |
, 2022
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/6345307 |
Daftar Isi:
- The raw time traces of the confined Brownian motion of 11 spores trapped within a 16 waveguide on-chip optical trap is visualized in the png files named "x(y)_timetrace_raw_1(2)". The respective Fourier spectrum and histogram of these time traces are named "Fourier_x(y)_raw_1(2)_dB" and "histogram_x(y)_raw_1(2)", respectively.. The raw time traces contain movement artifacts from complete image or chip displacement, which are removed by subtracting the tracked channel displacement from the tracked bead displacement. These time traces are named "x(y)_timetrace_channel_subtracted_1(2)". The result of subtracting the channel movement time traces is immediately visible in the respective Fourier transform, named "Fourier_x(y)_channel_subtracted_1(2)", through a dampening of the DC contribution and the contributions at 46 Hz and 72 Hz. The Gaussian histograms, named "histogram_x(y)_channel_subtracted_1(2)", on the other hand become narrower. Finally, as confined Brownian motion is governed by a Gaussian stochastic process the frequency spectrum is expected to be flat, where every frequency component contains the same energy. Consequentially, the remaining peaks at 46 Hz and 72 Hz are identified as noise artifacts and as such are filtered out. These filtered Fourier spectra are named "Fourier_x(y)_channel_subtracted_filtered_1(2)" are inverse Fourier transformed to obtain the final post processed time traces and histograms called "x(y)_timetrace_channel_subtracted_filtered_1(2)" and "histogram_x(y)_timetrace_channel_subtracted_filtered_1(2)". The Data and relevant matlab scripts for visualization are provided as attachments.