A simple paper chromatographic technique for detection of different types of histones in the unirradiated and gamma irradiated states in vitro
| Main Authors: | S. N. Upadhyay, Ram Pal Singh, V. S. Bhatnagar, H. C. Goel |
|---|---|
| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2002
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/5844481 |
Daftar Isi:
- Department of Radiation Chemistry, Department of Radiation Biology, Institute of Nuclear Medicine & Allied Sciences, Lucknow Marg, Delhi-110 054, India Fax: 91-011-3919505 Manuscript received 17 April 2001, revised 24 September 200/, accepted 12 November 2001 Paper chromatographic technique has been employed to detect low levels (20-25 μg/ml) of H1,H3 and H4 histones under physiological pH range in the native, heat denatured and gamma irradiated states (10-50 Gy). The Rf values obtained for these histones under different experimental conditions have rendered insight about the configurational alterations.