Green chemistry approach Synthesis of silver nanoparticles by using lime juice (Citrus aurantifolia) extract and their evaluation as antibacterial agent
| Main Authors: | Devendra Kumar, Neelam |
|---|---|
| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5733084 |
Daftar Isi:
- Department of Chemistry, Institute of Basic Sciences, Dr. B. R. Ambedkar University, Khandari Campus, Agra-282 002, Uttar Pradesh, India E-mail : devendrakumar131@gmail.com Manuscript received online 25 February 2014, revised 05 April 2014, accepted 08 April 2014 Nanoparticles of silver have been synthesized by the reaction of silver nitrate, lime juice (Citrus aurantifolia) and sodium bicarbonate by adopting green chemistry approach and characterized by FT-IR , UV-Visible spectral analysis, P-XRD, Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM) and elemental analysis by EDAX technique which revealed that silver nanoparticles were in metallic form. The average particle size was found 32 nm. They were screened for antibacterial activities on two gram +ve bacteria Staphylococcus aureus and Bacillus subtilis and two gram –ve bacteria Pseudomonas aeruginosa and Escherichia coli by adopting disc diffusion method in vitro. The results of antibacterial studies suggested that Ag nanoparticles can be used as effective growth inhibitors in microorganisms, making them applicable to medical devices and antimicrobial control systems.