Effect Of Intracanal Medicaments On RANKL and OPG Levels
| Main Author: | Lakshmi Thangavelu |
|---|---|
| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2021
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/5654263 |
Daftar Isi:
- The application of antimicrobial intracanal medication is recommended to remove bacteria that could not be removed from the root canal system after instrumentation and irrigation of the root canals. The aim of the present study was to evaluate the effects of calcium hydroxide alone and calcium hydroxide in combination with ibuprofen and ciprofloxacin on RANKL and OPG levels in periapical lesions cases. Thirty single rooted teeth with periapical lesions which require root canal treatment were randomly divided in to 2 groups; Group I- calcium hydroxide and Group II- calcium hydroxide with combination of ibuprofen and ciprofloxacin. After access opening and working length determination, interstitial fluid of the apical tissues was collected using paper points to assess the RANKL and OPG levels. Following which cleaning and shaping of the root canals were done and intracanal dressing was given (Group I n=15, Group II n=15). In the second appointment, medicaments were removed, and second sampling was performed using the same method. The RANKL and OPG levels were measured by the enzyme-linked immunosorbent assay, and the RANKL/OPG ratio was calculated. RANKL and OPG levels in pretreatment were Group I -0.75 pg/ml and 0.22 pg/ml and for Group II it was 0.72pg/ml and 0.2 pg/ml respectively. Post treatment RANKL and OPG were in group I 0.55 pg/ml and 0.67pg/ml and in group II it was 0.48 and 0.34pg/ml respectively. There was a significant difference between the groups in RANKL/OPG ratio, post treatment which showed RANKL and OPG ratio in the post treatment was less in Group I (0.8) compared with Group II (1.40) (p=0.02) (p < 0.05). With in the limitations of the study it proved that addition of ibuprofen and ciprofloxacin to calcium hydroxide did not have any extra benefit in lowering the RANKL/OPG ratio in periapical lesion cases.