Correlation between in vitro antioxidant activity and GC-MS evaluation of Calotropis gigantea (L.) R.Br. root extract
| Main Authors: | Sonia Singh, Shilpi Pathak, Nitin Agrawal, Partha Pratim Maiti |
|---|---|
| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5654185 |
Daftar Isi:
- Institute of Pharmaceutical Research, GLA University, Mathura-281 406, Uttar Pradesh, India Faculty of Pharmacy, Raja Balwant Singh Engineering Technical Campus Bichpuri, Agra-283 105, Uttar Pradesh, India Department of Pharmaceutical Technology, University of North Bengal, Darjeeling-734 014, West Bengal, India E-mail: sonia.singh@gla.ac.in Manuscript received online 22 October 2020, revised and accepted 10 November 2020 The present experiment was performed to evaluate the antioxidant potential and to identify the different bio-active components present in the methanol root extract of Calotropis gigantea. The preliminary phytochemical identification of Calotropis gigantea root extract was carried out, as to confirm the presence of primary and secondary metabolites. Antioxidant activity of methanolic root extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl and hydrogen peroxide radical scavenging assays. Phytochemical analysis revealed the presence of phenolic compounds, tannins, saponin, flavonoids, terpenoids, steroids and carbohydrates in root extract of Calotropis gigantea. Methanol root extract of C. gigantea possessed significant in vitro antioxidant activity when compared with ascorbic acid, as standard. And the GC-MS showed twelve chemical components present in methanol root extract of the plant. The above research concluded that the plant does contain different phytochemical components, especially phenolic compounds and flavonoids as of their pharmaceutical importance and become a crucial part in arresting the progression of several diseases and disorders.